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Although the Lab Manual describes it, here it is... Iris Diaphragm: used to regulate the amount of light entering the lens system. Coarse-adjustment knob: used to raise or lower the body tube of the microscope. Fine-adjustment knob: used to bring specimen into sharp focus. Condenser: controls the maximum numerical aperture and to move the condenser itself. Mechanical stage control: used to adjust the slide vertically and/or horizontally to bring slide into central opening of the objective.
Manipulate a MicroscopeInstructions1Set up the microscope on a flat surface. Always carry the microscope using two hands if the microscope must be transported from one area to another. One hand should support the bottom while the other hand is holding the arm. Once in place, remove the any covers and plug in the microscope.2Position the slide onto the stage. Clip it into or under the slide holders. Turn on the light from the illuminator under the stage. Center the slide the best way possible without using any focusing knobs or the eyepiece.3Look through the eyepiece, keeping both eyes open to avoid eye strain, while using the course focus knob to bring the stage and slide closer to the objective, which is the lens that extends below the eyepiece, until the object can be seen through the eyepiece. Use the fine focus knob (which will also be on the side of the microscope) to bring the image into sharper focus.4Center the object being viewed. Change the iris diaphragm under the stage using the level to open and close the iris. Change the amount of light entering the specimen as needed. Changing the amount of light going through the slide may show additional details not seen at higher light levels.5Change to the 10X objective which is the next longest lens by rotating the lens pieces. The object should appear larger and only require using the fine focus knob to bring it into sharper detail. Use the iris diaphragm to change the amount of light entering the slide. Continue to the 40X objective and repeat. Each time the object will appear larger and you will be able to see less of the object at any one time while viewing.6Lower the stage and add one or two drops of immersion oil to the slide. Change the microscope to the 100X objective. Slowly raise the stage using the course focus until the objective is touching the oil. Use the fine focus knob to bring the object into sharp focus. Clean the slide and objective with lens tissue after use.7Return the stage to the lowest level. Change the objective back to the 4X lens. Unplug the machine and replace any covers. Carefully return the microscope using both hands to where it is stored.
You may mean: 1. focus, the place where the rays from a lens or mirror all cross at a point; the place where an image appears sharp; 2. fucose, a type of de-oxy sugar involved in cell biology.
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there are many names for this explanation and a particular name is..............
use the fine focus knob until we get sharp image
To bring a sharp focus.
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
For the adjustment of focus, you would have to use the Fine-adjustment knob located on the left and right sides of the microscope arm.
Because it is too hard to find a specimen on high power. Easier to find them and focus them on low power and then increase the magnification and fine-tune the focus to get a better, closer look.
Auto Focus
It's called the "working distance" and is defined as, "the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus."
it can do to the singurlity of a blackhole.
The depth of field is the part of a specimen that is in sharp focus; the depth of field decreases as the NA increases. The depth of focus on the other hand is the magnified image in focus on the film plane; depth of focus decreases as magnification increases. To learn more about microscopes and its uses visit the website in the link below.
Although the Lab Manual describes it, here it is... Iris Diaphragm: used to regulate the amount of light entering the lens system. Coarse-adjustment knob: used to raise or lower the body tube of the microscope. Fine-adjustment knob: used to bring specimen into sharp focus. Condenser: controls the maximum numerical aperture and to move the condenser itself. Mechanical stage control: used to adjust the slide vertically and/or horizontally to bring slide into central opening of the objective.
A concave mirror will tend to "focus" light to some degree, depending on the curvature of the mirror. A parabolic mirror will bring reflected light to a sharp focus, and telescopes use this type of curve for the shape of their reflective surface. And for exactly the reasons you think they do. Want links? You got 'em....
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