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What are the research tools that were used in the past and are use today to study DNA structure and function?
DNA atomic structure - X-ray crystallography DNA base sequencing - Polymerase Chain Reaction in conjunction with gel electrophoresis. DNA function - splicing DNA fragments into plasmids, then infecting host bacteria. Other methods include heredity studies. J Ayres
Why do tiny bubbles form on and amongst DNA strands during extraction?
When the detergent/salt/DNA mixture is agitated, the detergent, along with some inadvertently trapped gas, forms bubbles, and these bubbles may stick to the DNA and the histone proteins. They are not formed by any chemical reaction.
What enzyme joins the nucleotides of a DNA strand together?
That depends on the process. During DNA replication, The nucleotides of the lagging strand (Okazaki fragments) are connected by DNA ligase. In transcription, the nucleotides of RNA are connected by RNA polymerase II.DNA Polymerse
How could you analyze the DNA once it is extracted?
Forensic scientists can use DNA in blood, semen, skin, saliva or hair found at a crime scene to identify a matching DNA of an individual, such as a perpetrator. This process is formally termed DNA profiling, but may also be called "genetic fingerprinting".In DNA profiling, the lengths of variable sections of repetitive DNA, such as short tandem repeatsand minisatellites, are compared between people. This method is usually an extremely reliable technique for identifying a matching DNA.
How are traits passed from parents to their offspring?
Traits are passed by DNA.
What substrates are used in the DNA synthesis reaction?
The substrates used in the DNA synthesis reaction are deoxyribonucleoside triphosphates (dNTPs), which are the building blocks of DNA.
Which process uses bacteria to copy DNA?
Polymerase chain reaction
What is the product of a DNA synthesis reaction?
The product of a DNA synthesis reaction is a newly synthesized DNA strand complementary to the template DNA strand. This process involves the incorporation of nucleotides by DNA polymerase to form a double-stranded DNA molecule.
What reaction is used to radioactively label DNA?
The reaction used to radioactively label DNA is typically performed using a DNA polymerase enzyme along with radioactive nucleotides, such as [α-32P]dNTPs. This allows for the incorporation of the radioactive label into the DNA strand during the polymerase reaction.
What the price for which promise of the other is bought?
It is called consideration.
How do you set up a PCR reaction?
To set up a PCR reaction, you mix together DNA template, primers, nucleotides, DNA polymerase, and buffer in a tube. Then, you run the reaction through a series of temperature cycles in a thermal cycler to amplify the DNA.
Which is used to copy DNA for DNA fingerprinting?
PCR
What is another term for synthesis reaction?
DNA synthesis is also known as DNA replication.
What is the definition of Promisor demands and receives as the price for a promise?
executor
What is the correct sequence of events that occur in a PCR reaction?
In a PCR reaction, the correct sequence of events is denaturation, annealing, and extension. Denaturation involves heating the DNA to separate the strands. Annealing involves cooling the reaction so primers can bind to the DNA. Extension involves DNA polymerase synthesizing a new strand of DNA using the primers as templates.
What are the key steps involved in performing a successful one primer PCR reaction?
The key steps in performing a successful one primer PCR reaction include: preparing the reaction mix with the primer, template DNA, nucleotides, and polymerase; denaturing the DNA at a high temperature; annealing the primer to the template DNA at a lower temperature; and extending the primer to create new DNA strands. The reaction is then cycled through these steps multiple times to amplify the target DNA.
What kind of reaction is DNA replication and what does it require?
DNA replication is a semi-conservative process where a DNA molecule makes a copy of itself. It requires enzymes such as DNA polymerase, dNTPs (deoxynucleotide triphosphates), a template DNA strand, and primer to initiate the process.
