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The Enterotube test, while convenient for identifying enteric bacteria, has several disadvantages. It may provide false positives or negatives due to the complex biochemical reactions involved, leading to misidentification. Additionally, the test is limited in its ability to differentiate closely related species and may not cover all possible pathogens. Furthermore, it requires a certain level of expertise to interpret the results accurately, which can be a barrier in some settings.

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1w ago

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Can the Enterotube II be used on any unknown organism?

The Enterotube II system is designed for the identification of Enterobacteriaceae, which are a specific family of gram-negative bacteria. It may not be suitable for identifying unknown organisms outside of this family.


Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?

The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.


What are the advantages and disadvantages of the scientific method?

Advantages of using scientific methods: You can test in many different ways, and the more times you test, the more accurate your results will be. Disadvantages of using scientific methods: You can come up with different results which can disprove your hypothesis, and this leads to inconsistent conclusions.


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Why shoud the first digit in the enterotube II code always be equal to or greater than 2?

The Enterotube II System is generally used to distinguish between Enterobacteriaceae. Members of this family are generally glucose fermenters and some also produce gas. Since you get a "2" for glucose fermentation and a "1" for gas production, the value of the first number should be 2 or 3 in order to identify an organism using the Enterotube II System.


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Which clinical specimen is used for enterotube inoculation?

stool or feces


Would you try to identify a Gram positive coccus using the Enterotube II?

The Enterotube II is not typically used to identify Gram-positive cocci. It is primarily used to identify and differentiate Enterobacteriaceae - a family of Gram-negative bacteria commonly found in the intestines. To identify Gram-positive cocci, other tests such as catalase, coagulase, and hemolysis tests are more appropriate.


How does an enterotube work?

An Enterotube is a multiple-test system used to identify different species of Enterobacteriaceae based on their biochemical reactions. The tube contains compartments with specific biochemical tests, and when inoculated with a bacterial sample, the reactions are observed to identify the species present. Results are interpreted based on color changes or gas production in each compartment to determine the identity of the bacteria.


What is the purpose of Enterotube?

Enterotube is a system used in microbiology to identify and differentiate enteric bacteria based on their biochemical characteristics. It consists of multiple compartments containing various substrates that can be utilized by the bacteria for metabolic reactions, helping to identify the species present. Enterotube allows for rapid and accurate identification of bacteria in clinical and research settings.


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