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An Enterotube is a multiple-test system used to identify different species of Enterobacteriaceae based on their biochemical reactions. The tube contains compartments with specific biochemical tests, and when inoculated with a bacterial sample, the reactions are observed to identify the species present. Results are interpreted based on color changes or gas production in each compartment to determine the identity of the bacteria.
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Which clinical specimen is used for enterotube inoculation?
stool or feces
What is the purpose of Enterotube?
Enterotube is a system used in microbiology to identify and differentiate enteric bacteria based on their biochemical characteristics. It consists of multiple compartments containing various substrates that can be utilized by the bacteria for metabolic reactions, helping to identify the species present. Enterotube allows for rapid and accurate identification of bacteria in clinical and research settings.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
Would you try to identify a Gram positive coccus using the Enterotube II?
The Enterotube II is not typically used to identify Gram-positive cocci. It is primarily used to identify and differentiate Enterobacteriaceae - a family of Gram-negative bacteria commonly found in the intestines. To identify Gram-positive cocci, other tests such as catalase, coagulase, and hemolysis tests are more appropriate.
What is the advantage of API 20E system over the Enterotube II systeme?
API 20E system is advantageous over Enterotube II system for bacterial identification in clinical laboratories because it can provide a more comprehensive identification of Enterobacteriaceae and non-fermenting gram-negative bacteria. It is also known for its user-friendly format and interpretation system, making it easier for technicians to use. Additionally, API 20E system is more standardized and has a higher accuracy rate compared to the Enterotube II system.
Which clinical specimen is used for enterotube inoculation?
stool or feces
What is the purpose of Enterotube?
Enterotube is a system used in microbiology to identify and differentiate enteric bacteria based on their biochemical characteristics. It consists of multiple compartments containing various substrates that can be utilized by the bacteria for metabolic reactions, helping to identify the species present. Enterotube allows for rapid and accurate identification of bacteria in clinical and research settings.
Can the Enterotube II be used on any unknown organism?
The Enterotube II system is designed for the identification of Enterobacteriaceae, which are a specific family of gram-negative bacteria. It may not be suitable for identifying unknown organisms outside of this family.
Why shoud the first digit in the enterotube II code always be equal to or greater than 2?
The Enterotube II System is generally used to distinguish between Enterobacteriaceae. Members of this family are generally glucose fermenters and some also produce gas. Since you get a "2" for glucose fermentation and a "1" for gas production, the value of the first number should be 2 or 3 in order to identify an organism using the Enterotube II System.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
What is an Enterotube?
An Enterotube is a rapid diagnostic tool used in microbiology to identify and differentiate Enterobacteriaceae bacteria based on their metabolic properties. It contains multiple compartments filled with specific substrates, allowing the bacteria to ferment and produce characteristic color changes that can be used to identify the specific species. Enterotubes are commonly used in clinical laboratories to quickly identify bacteria from clinical samples.
Would you try to identify a Gram positive coccus using the Enterotube II?
The Enterotube II is not typically used to identify Gram-positive cocci. It is primarily used to identify and differentiate Enterobacteriaceae - a family of Gram-negative bacteria commonly found in the intestines. To identify Gram-positive cocci, other tests such as catalase, coagulase, and hemolysis tests are more appropriate.
What is the advantage of API 20E system over the Enterotube II systeme?
API 20E system is advantageous over Enterotube II system for bacterial identification in clinical laboratories because it can provide a more comprehensive identification of Enterobacteriaceae and non-fermenting gram-negative bacteria. It is also known for its user-friendly format and interpretation system, making it easier for technicians to use. Additionally, API 20E system is more standardized and has a higher accuracy rate compared to the Enterotube II system.
What are disadvantages of using Enterotube test?
The Enterotube test, while convenient for identifying enteric bacteria, has several disadvantages. It may provide false positives or negatives due to the complex biochemical reactions involved, leading to misidentification. Additionally, the test is limited in its ability to differentiate closely related species and may not cover all possible pathogens. Furthermore, it requires a certain level of expertise to interpret the results accurately, which can be a barrier in some settings.
What is your bacteria if your enterotube results are 3 2 1 6 1?
Based on the enterotube results of 3 2 1 6 1, your bacteria is likely Enterobacter cloacae. Each number corresponds to a specific biochemical test, and the pattern matches the biochemical reactions of E. cloacae. This organism is commonly found in the environment and can cause infections in humans.
Why is pure culture necessary for enterotube tests but not for DNA probes?
For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.
Why should the first digit in the five-digit Enterotube II ID value be equal to or greater than 2?
The first digit in the five-digit Enterotube II ID value being equal to or greater than 2 is essential because it indicates that the test is designed for the identification of Enterobacteriaceae and related organisms. Values of 1 or lower are reserved for other types of tests or categories that do not pertain to this specific group. This ensures accurate identification and classification, maintaining the integrity of the testing system.
