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Can the Enterotube II be used on any unknown organism?
The Enterotube II system is designed for the identification of Enterobacteriaceae, which are a specific family of gram-negative bacteria. It may not be suitable for identifying unknown organisms outside of this family.
How is it possible for the same species to have two or more identification codess with the enterotube?
Not all bacteria of a given species will produce the same result in a given test media. For example, according to Bergey's, somewhere between 11% and 89% of E. coli are positive for ornithine decarboxylase. Since this test is inconclusive either a positive or negative result is acceptable if other tests indicate that the species being tested is E. coli.
Which clinical specimen is used for enterotube inoculation?
stool or feces
What is the purpose of Enterotube?
Enterotube is a system used in microbiology to identify and differentiate enteric bacteria based on their biochemical characteristics. It consists of multiple compartments containing various substrates that can be utilized by the bacteria for metabolic reactions, helping to identify the species present. Enterotube allows for rapid and accurate identification of bacteria in clinical and research settings.
Can the Enterotube II be used on any unknown organism?
The Enterotube II system is designed for the identification of Enterobacteriaceae, which are a specific family of gram-negative bacteria. It may not be suitable for identifying unknown organisms outside of this family.
Why shoud the first digit in the enterotube II code always be equal to or greater than 2?
The Enterotube II System is generally used to distinguish between Enterobacteriaceae. Members of this family are generally glucose fermenters and some also produce gas. Since you get a "2" for glucose fermentation and a "1" for gas production, the value of the first number should be 2 or 3 in order to identify an organism using the Enterotube II System.
Would you try to identify a Gram positive coccus using the Enterotube II?
The Enterotube II is not typically used to identify Gram-positive cocci. It is primarily used to identify and differentiate Enterobacteriaceae - a family of Gram-negative bacteria commonly found in the intestines. To identify Gram-positive cocci, other tests such as catalase, coagulase, and hemolysis tests are more appropriate.
What is the advantage of API 20E system over the Enterotube II systeme?
API 20E system is advantageous over Enterotube II system for bacterial identification in clinical laboratories because it can provide a more comprehensive identification of Enterobacteriaceae and non-fermenting gram-negative bacteria. It is also known for its user-friendly format and interpretation system, making it easier for technicians to use. Additionally, API 20E system is more standardized and has a higher accuracy rate compared to the Enterotube II system.
How does an enterotube work?
An Enterotube is a multiple-test system used to identify different species of Enterobacteriaceae based on their biochemical reactions. The tube contains compartments with specific biochemical tests, and when inoculated with a bacterial sample, the reactions are observed to identify the species present. Results are interpreted based on color changes or gas production in each compartment to determine the identity of the bacteria.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
What is your bacteria if your enterotube results are 3 2 1 6 1?
Based on the enterotube results of 3 2 1 6 1, your bacteria is likely Enterobacter cloacae. Each number corresponds to a specific biochemical test, and the pattern matches the biochemical reactions of E. cloacae. This organism is commonly found in the environment and can cause infections in humans.
Why is pure culture necessary for enterotube tests but not for DNA probes?
Pure culture is necessary for enterotube tests to ensure accurate identification of bacterial species based on their biochemical characteristics. In contrast, DNA probes target specific genetic sequences, allowing for identification of pathogens even in mixed cultures without the need for isolation. DNA probes rely on the presence of the target sequence rather than the phenotype of the microorganism.
Why should the first digit in the five-digit Enterotube II ID value be equal to or greater than 2?
The first digit in the five-digit Enterotube II ID value being equal to or greater than 2 is essential because it indicates that the test is designed for the identification of Enterobacteriaceae and related organisms. Values of 1 or lower are reserved for other types of tests or categories that do not pertain to this specific group. This ensures accurate identification and classification, maintaining the integrity of the testing system.
Why should the first digit in the five-digit Enterotube ID value be equal to or greater than 2?
The first digit in the five-digit Enterotube ID value must be equal to or greater than 2 to ensure that the identification process is consistent and reliable. This requirement typically indicates that the organism being tested falls within a certain category of Enterobacteriaceae, which is essential for accurate classification and differentiation of enteric bacteria. Using a range starting from 2 helps avoid confusion with other non-enteric organisms and aligns with established identification protocols.
