0
What are the advantages and disadvantages of pour plate method?
Wiki User
∙ 15y ago
Advantages:
1. Counts only living cells
2. Standardized test - used worldwide
Disadvantages:
1. 1-2 days of incubation
2. Melted, heated agar
3. Osmotic shock
Wiki User
∙ 15y ago
Wiki User
∙ 11y ago1. An inoculated plate is always incubated in an inverted position to prevent condensation from falling onto the surface of the plate and interfering with discrete colony formation.
2. Do not allow any agar to splash over the side of the plate.
Add your answer:
Earn +20 pts
Could some bacteria grow on the streak plate and not be seen using the pour plate technique?
Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Compare the pour plate and streak plate methods of isolating bacteria?
I got the answer but you hav ta do your labreport yourself..haha Whoever wrote this is an a$$hole. I wish that you went to my school so I could kick your aXX..Ha ha back at you, jerk! thanks jerk,,that was really helpful.. anyway guys, here is the correct and precise answer, hope it will help u to complete ur lab repor..:) In streak plate method, After the first sector is streaked in dish, the inoculating loop is sterilized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cell will drop from it as it is rubbed along the agar surface. These develop into separate colonies. Whereas, in the pour plate culture, sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. After the agar has hardened each cell is fixed in place and forms an individual colony.
How long does it take for one milliliter to evaporate?
Depends on the rate of supply of heat and the relative humidity. It could take forever (100% relative humidity), or be almost instantaneous (pour onto a red hot plate with < 100% humidity).
How could the pour plate procedure be used for counting the number of bacteria in a sample?
It is recommended that plates that should be counted should be between 30 and 300 McCance and Harrigan (1992). When more that 300 colonies you cant count them with great accuracy and represented with TNC ( too numerous to count). If colonies are less that 30 do you conclude that there was no bacteria in a product or food, if counting less than 20 colonies is unrealistic. Some standards are zero tolerant where even one colony means alot. So IDF (19991/1992) came up with a formula which takes in account the plates with/ even less than 30 colonies. The formula takes into account all sums of colonies obtained on the pour plates to come up with total cfus
What advantages does the streak-plate method have over the pour plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What advantages does the pour plate method have over streak plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
Does the streak plate method work better than the pour plate method?
I 4 got
How do the results of pour plate method compare with those obtained using the streak plate and spread plate method?
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
What is the method called when the bacteria sample and nutrient agar are mixed before pouring?
Pour plate method
Do both the spread-plate and pour-plate method in a experiment produce similar bacterial counts or are they vastly different?
Both Spread-plate and pour plate method don't give the same results. Because in the case of spread plate method the inoculmn used for inoculation can't be spread in a exact volume. A little inoculmn remains stick with the spreader after spreading. On the other hand, in pour plate method it doesn't happen. So mostly, through comparing the counts by both methods, less counts are obtained in spread plate method. I am Working as a Sr. Microbiologist in a Biotech company
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
Which method often results in colonies developing down throughout the agar and some colonies on the surface?
pour plate
How do you detect acid and gas production using solid medium?
By using pour-plate method with the suitable growth medium.
Under what kind of plating technique would you find deep or buried colonies?
In pour plate method, in this method of plating or culturing the microbial sample first diluted and pour in empty plate and after that growth media is poured in it and which is then skake firmly. As the microbial suspension or sample is distributed in media the growth of bacteria occurs in buried of deep positions.
What is the main advantage of pour-plate method over other methods of bacterial colony isolation?
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
How do you identify a contamination on a pour plate?
You can identify a contamination on a pour plate by color, size, shape, texture, or growth rate.
