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Ok, it's 1.5 mg per mL of Blood, you can reach this concentration by disolving 0.6 mg of EDTA in 10 mL of still water, then you add 100 microLitters (0.1mL) of this 6% EDTA into a glass tube. The next thing you've to do is to dry the test tube so that you'll only have 6 mg of EDTA, enough to anticoagulate exactly 4 mL of fresh complete blood. The final concentration of EDTA in the blood should be into the range of 1.25 to 1.75 mg per mL. I found this in Dacie's Haematology. karlosgb@live.com.mx
What else can I help you with?
What color lab tube is used for ESR?
Lavender (EDTA) top tube. Hyperlipidemic or hyperbilirubinemic samples should not be tested. - excerpted from Bakerman's ABC's
What kind of tube is used for CBC?
It all depends on what system your using. If by tube you are referring to vaccutainers (blood sample containers) then you have to check. I know that purple top (EDTA) is for the ABC CBC machine as well as a manual blood smear since you need whole blood and EDTA minimizes any distortions.
What is the full name for te buffer?
It is a buffer used in biology. "te" is derived from its components: t from tris, a common pH buffer, and e from the EDTA, a molecule. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
What effects does the concentration of reactants have on the rate of the reaction?
increasing the concentration increases the rate of the reaction
Can you titrate a solution of unknown concentration with another solution of unknown concentration and still get a meaningful answer?
yes you can, however you need the chemical coumpound name, how many grams of that chemical compound was used, and the litres that it was mixed with. for example: 2g of NaF was used to mix in a 1L solution (NaF has a molas mass of about 42g) 2g of NaF x 1 mol/42g (grams cancel and left with mols) 0.048mol/1L = 0.048mol/L conentartion
What preservatives are used in mayonnaise with what level of concentration?
EDTA
What is the purpose of standardizing EDTA in Complexometric titration?
Standardizing EDTA in complexometric titration is done to determine its exact molarity or concentration. This is important because the accuracy of the titration results depends on knowing the precise concentration of the EDTA solution being used. By standardizing EDTA, any errors in concentration can be corrected, ensuring accurate and reliable results in the titration process.
How do you calculate the concentration of EDTA in titration?
in order to titrate a sample of solution, lets take an example. If we have a solution of 1.569 mg of Coso4, which has a (155.0g/mol ratio) per mill. A question may ask us to find the volume of Edta needed of titrate an aliqout of this solution. So lets take a random number of 0.007840 M EDTA and be asked to titrate A 25.00ML Aliqout of this solution. How do we find the volume of EDTA needed.....? well first we use the numbers given, 1.569 mg CoSo4/ ml x (1g/1000mg)(1molcoso4/155.0g)(1molEDTA/1mol CoSo4) calculating this out should give 1.012 x 10 ^-5 mol of EDTA per ml. we then multuply the moles of EDTA which react with 1.569 ml of COso4 by 25.00 ml 1.012x10^-5 mol edta (25.00ml)= 2.531 x 10^-4 mol of edta. This is the amount of moles in the new solution. Now we need to find the amount of moles per liter of the specific concentration of EDTA. so we multiply 2.531x10^-4 mol edta x (1L/0.007840 mol) to give 0.03228 Liters of 32.28 ml .
How does EDTA and titration work?
EDTA is a chelating agent that binds to metal ions. In titration, EDTA is used to determine the concentration of metal ions in a solution by forming a complex with the metal ion. The endpoint of the titration is identified by a color change indicator or a pH meter, indicating that all metal ions have reacted with EDTA.
What kind of reaction take place between the calcium and EDTA?
The reaction between calcium and EDTA is a complexation reaction in which the EDTA molecule binds to the calcium ion, forming a stable, water-soluble complex. This reaction is used in titrations to determine the concentration of calcium in a sample.
What is the final concentration of a edta solution if a 1l of 50x stock solution was made using 18.612g of edta mw 372.24?
the mass of the EDTA used to prepare 1L oi solution is 18.612g. The formula mass is 372.24 g/mol. Therefore concentration of the 50x solution is: 18.612 g 1 mol ----------- x --------- = 0.050000 mol/L = 50.000 mM 1L 372.24 g
What is the function of hhsnna in edta titration?
In EDTA titration, hhsnna (hydroxylamine hydrochloride) is used to reduce any interfering metal ions present in the sample to prevent their titration by the EDTA solution. This helps ensure that the titration results are accurate and only reflect the concentration of the target metal ion being measured.
How you can do EDTA titration reaction?
To perform an EDTA titration, first prepare a solution containing the analyte (the substance being measured) and a suitable indicator, such as Eriochrome Black T. Add a standardized solution of EDTA to the analyte solution until the endpoint is reached, indicated by a color change in the indicator. The volume of EDTA solution added can be used to calculate the concentration of the analyte based on the stoichiometry of the reaction.
What are important applications of EDTA titrations?
Important applications of EDTA titrations include determining the concentration of metal ions in solution, such as calcium or magnesium in water samples, and determining water hardness. EDTA titrations are also used in the pharmaceutical industry to analyze the purity of drug compounds and in food industry to measure metal ions in food samples.
How do you measure permanent hardness by EDIT titration?
To measure permanent hardness by EDTA titration, first add a buffer solution to the water sample to maintain a stable pH. Then, titrate with standardized EDTA solution until the color changes indicating the endpoint. The volume of EDTA required to reach the endpoint can be used to calculate the concentration of the ions causing permanent hardness in the water.
Determination of fe3 with EDTA by photometric titration?
Iron (III) ions form a deep-coloured complex with a maximum absorption at about 525nm; this complex is used as the basis for the photometric titration of iron(III) ion with standard EDTA solution.
What is disodium edta?
Used in solarcaine.
