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Inoculum size refers to the quantity or concentration of microorganisms introduced into a culture or environment to initiate growth or fermentation. It is a critical factor in microbiology and biotechnology, as it can influence the speed and efficiency of microbial growth, metabolic activity, and the final yield of desired products. An optimal inoculum size ensures a successful fermentation process and can vary depending on the type of organism and the specific application.
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Why cooling the inoculating instrument prior to obtaining the inoculum?
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
Explain why a false positive reaction result can be obtained with Koser's citrate medium?
If carbon sources, or nutrients, are carried over into the citrate medium, it will result in a false positive. To avoid this, dilute the inoculum in saline before inoculating the citrate medium.
What should be the size of a plane mirror to get a full size image of a person?
The size of the plan mirror should be half the size of the object to get a full size image of the object
What is a size 7 in inches?
size 7 in inches
Does the size of the fruit effect the voltage?
The size of the fruit does not effect the voltage. If you're making a light, the size can effect how long it lasts. The greater the size, the longer it will last
The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the?
Inoculum
What factors must be carefully controlled in the kirby-Bauer method?
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
What factors must be carefully control in the Kirby-Bauer method?
purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.
The small amount of growth on the end of an inoculating loop is called an?
Inoculum
What factors may be the cause for an extended lag phase in a bacteria groth curve?
Factors that may contribute to an extended lag phase in a bacterial growth curve include: suboptimal environmental conditions (such as pH, temperature); competition for nutrients or space with other microorganisms in the culture; prior exposure to stressors or antibiotics; mutations affecting growth rate; or the need for bacteria to adapt to a new environment or substrate.
Why cooling the inoculating instrument prior to obtaining the inoculum?
The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.
What are the advantages and disadvantages of multitest system for bacterial identification?
the obvious advantages of these units are the need for minimal storage space,the use of less media, the rapidity with which results may be obtained, and the applicability of the results to a computerized dystem for identification of organisms. disaadvantage including difficulty in obtaining the proper inoculum size since some media require heavy inoculation while others need to be lightly inoculated,possibility of media carryover from one compartment to anther,using inoculum of improper age.
Why loop and cap should not be laid on workbench while transferring inoculum?
To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.
What is the optimum temperature for inoculum?
The optimum temprature is that temprature at which bacteria grows and multiply at its full extent, because of its favaurable conditions avialable.
What is the best way to hold the upper slide when using it to spread the inoculum across the slide being stained?
At a 45 degree angle
Why inoculum is take from edge not from center?
The bacteria on the margin are the most active and healthiest because they have access to fresh nutrients and have not yet contaminated the culture medium with waste.
What is the consequence of not spreading the inoculum adequately over the agar surface?
Not spreading the inoculum adequately over the agar surface can lead to inaccurate results in microbiological studies. Uneven distribution may result in uneven growth of microbial colonies, making it difficult to accurately interpret and analyze the data. This can impact the reliability and reproducibility of experimental outcomes.
