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What is th blue dye added to the sample before the electrophoresis is performed?
Wiki User
∙ 15y ago
In biochemistry labs, the traditional answer for a protein gel (polacrylamide gel electrophoresis) is bromphenol blue. For a DNA gel (agarose gel electrophoresis), traditionally the same dark blue dye bromphenol blue was combined with the lighter, slower migrating blue dye xylene cyanol.
Oftentimes nowwe only use the bromphenol blue, or even substitute for it with Orange G, which is a UV-transparent dye that more easily enables the visualization of smaller molecular weight nucleic acids that migrate in the same region.
Wiki User
∙ 15y ago
Wiki User
∙ 14y agoIn electrophoresis, stains or dyes are often needed in order for the bands to be visible in the gel.
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What will be the colour of the universal indicator if it is added to a sample of egg yolk?
green.
Why is the milk cooled before the bacteria are added?
Because it is. face it
The specific heat of substance A is greater than that for substance B If both sample sizes are the same and they both start at the same temperature and equal amounts of heat are added to both these s?
The specific heat of substance A is greater than that for substance B. If both sample sizes are the same and they both start at the same temperature and equal amounts of heat are added to both these samples, substance A will have a lower temperature than substance B.
How is plastic dyed?
Before the plastic is molded dyes are added to the molten plastic.
How does a can of paint get its color?
Pigments are added to the paint. Pigments are added to the paint. Pigments are added to the paint.
What is the function of the blue dye added to the sample before the electrophoreses is performed?
The blue dye is usually a combination of glycerol and something else. But I believe the most important part is the glycerol. Glycerol is heavier than the buffer that you actually perform the electrophoresis in.By adding the glycerol to your sample, you give it weight so that it doesn't float around when you're trying to pipette it into your well and so that it will just fall.
What happen to these protein when the sample is added to the wells of the microscope strip?
the proteins will go away when the sample is added
Was the water in a cylinder before the sample was added?
after 5.63 gm sample of wood metal was added in a 10ml graduated cylinder the new water level is 8.7ml "http://wiki.answers.com/Q/Was_the_water_in_a_cylinder_before_the_sample_was_added" after 5.63 gm sample of wood metal was added in a 10ml graduated cylinder the new water level is 8.7ml "http://wiki.answers.com/Q/Was_the_water_in_a_cylinder_before_the_sample_was_added"
Why Na2CO3 is added to solution before Benedicts test is performed?
Sodium carbonate is added with the purpose to increase the pH of the solution.
Why is sodium carbonate added to solution before Benedicts test is performed?
Sodium carbonate is added to increase the pH of the solution.
What is used to make the DNA visible on the gel?
YES!! You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp. If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.
How can gel electrophoresis be used to establish parentage?
Gel electrophoresis is used to separate substances in a sample by applying a small electrical charge. The positively charged substances will gravitate one direction based on the amount of the moleular charge and the negatively charged substances will gravitate in the other direction based on it's charge. DNA will move away from the negative anode towards the positively charged cathode and will separate based on it's length. A stain is added to the gel separated DNA to make it visible to the human eye and then it is compared to a known sample to establish forensic (criminal) match or a similar pattern for parentage.
Why are the loading dye added to the samples before they are loaded into the wells?
The loading dye is added to the samples before they go into the wells, because it increases the density enough to make the samples sink to the bottom of the wells. A sample of DNA that contains residual ethanol when it is placed in the well may float.
When 5.10 kJ of heat energy is added to a 430 g sample of silver?
When 5.1 kJ of heat energy is added to a 430 g sample of silver, find specific heat of Ag
What is the production of a gas when acid is added to a sample of chalk is an indication?
chemical
What will be the colour of the universal indicator if it is added to a sample of egg yolk?
green.
What will happen to the COD value if HgSO4 is not added?
Chlorine will not be seperated out of the sample effluent.
