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excess liquid on paper disk leads to unequal radial diffusion

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How to determine whether zone of inhibition is due to death of the bacteria or its inhibition?

If the compound you are testing is bacteriocidal, you will not be able to recover bacteria from the zone of inhibition. If the compound is only bacteriostatic, you should be able to recover bacteria from the zone of inhibition by scraping the surface of the agar and resupending the scrapings in sterile saline and then spreading an aliquot of the suspension on nutrient agar that does not contain the bacteriosttic agent.


What is the differences between zone of inhibition and zone of exhibition?

Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-


Why is a larger zone of inhibition better than a small zone of inhibition?

A larger zone of inhibition means that the applied agent has either inhibited or killed the organisms that were spread on the plate and that those organisms are susceptible to that agent. In other words, a larger zone of inhibition means that the applied agent is more effective in killing/inhibiting the bacteria around it.


How can you determine whether the zone of inhibition is due to death of a bacterium or to inhibition of growth?

To determine whether the zone of inhibition is due to death of a bacterium or inhibition of growth, you can perform a viability assay by transferring a sample from the zone to a growth medium and observing if growth occurs. If growth does not occur, it suggests bactericidal activity. Additionally, conducting time-kill studies can help differentiate between bacteriostatic and bactericidal effects based on the rate and extent of bacterial killing over time.


What is the purpose of inoculating tsa plates with samples from the zone of inhibition?

Any bacteria that grow in the zone of inhibition are resistant to the antibacterial used. By inoculating new plates with the bacteria, you will have a pure culture of resistant bacteria.


Does the edge of zone inhibition directly indicate the limit of bacitracin?

Yes, the edge of the zone of inhibition directly indicates the limit of where the bacitracin has prevented bacterial growth. The zone of inhibition represents the area around the antibiotic disk where bacterial growth has been suppressed, providing a visual indication of the effectiveness of the antibiotic against the tested bacteria.


Is antibiotic beyond the zone of inhibition?

the area (zone) in which bacteria cannot grow due to the presence of an antibiotic paper disk


How does the thickness of agar affect the diameter of the zone of inhibition?

A thicker layer of agar may lead to a larger zone of inhibition due to the increased diffusion distance for the antimicrobial agent. The agar thickness can influence the rate of diffusion of the antimicrobial agent into the agar, impacting the size of the zone of inhibition formed around the antimicrobial disk.


Why some colony grow inside the zone of inhibition?

Some bacteria within the colony may possess inherent resistance to the antibiotic, allowing them to survive and grow within the zone of inhibition. Additionally, mutations in the bacterial DNA can confer resistance, enabling the growth of colonies within the zone. Horizontal gene transfer mechanisms like conjugation can also introduce resistant genes to susceptible bacteria in the colony, leading to their growth within the zone of inhibition.


If a petri dish shows a large zone of inhibition what does this say about the antibiotic?

A large zone of inhibition on a petri dish indicates that the antibiotic has effectively inhibited the growth of bacteria surrounding the disc. This suggests that the antibiotic is potent against the bacteria being tested, as it can effectively prevent their growth. A larger zone of inhibition usually indicates a higher concentration of antibiotic or greater efficacy against the bacteria.


How should the zone of inhibition be measured in microbiology experiments?

The zone of inhibition in microbiology experiments should be measured by using a ruler to measure the diameter of the clear area around the antimicrobial disk. This measurement helps determine the effectiveness of the antimicrobial agent against the bacteria.


What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?

This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.