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You can make a PH indicator solution out of blueberry juice, or you can buy litmus paper or use an indicator solution that you can buy at the store.
Touch it to the sterile agar in a plate. If it melts the agar, it is still hot. If it doesn't melt the agar, then it is cool enough.
The size of the molecules.
Non-selective is when we use agar for bacterial growth in general. Mean not to differentiate between them but just to see their colonies and growth. TSA and Chocolate agar are nonselective.
When you mix agar and dissolved silver nitrate, the silver ions in the silver nitrate solution can bind to the agar molecules, forming a complex. This complexation may result in the precipitation of silver agar particles, depending on the concentration of silver ions and the pH of the solution.
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800ml H2O10g Bacto-tryptone5g yeast extract10g NaClAdjust pH to 7.5 with NaOHAdd 15g agarMelt agar into solution in the microwaveAdjust volume to 1L with dH2OSterilize by autoclaving
Agar is a gelatinous substance extracted from seaweed. One of its many uses is to solidify nutrient broth used in growing bacteria on a petri dish. A petri dish is a two piece clear glass or plastic dish which keeps dirt or dust from falling into the sterile medium, but allows air to enter and carbon dioxide to leave.
It allows the agar to cool, if the agar is too hot it could kill the bacteria if you are pouring it right onto a sample, if you are pouring it into plates to use later it just makes it easier to pour as you will not be burning your hand off
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Nutrient agar typically solidifies within 1-2 hours after being prepared and poured into a petri dish. The solidification time can vary depending on room temperature and the specific formulation of the agar medium.
A local market or bakery.
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
The universal indicator in agar solution can change color based on the pH of the solution. At neutral pH (around 7), it usually appears green. In acidic conditions, the color may shift towards shades of red/pink, while in alkaline conditions, it may turn to shades of blue/purple.
Top agar is a type of agar medium used in microbiology to perform overlay bacterial or phage assays. It consists of a lower layer of solid nutrient agar covered by a thin layer of softer agar on top, which allows for the diffusion of substances while providing a supportive surface for the growth of microorganisms. This technique is commonly used to detect bacterial or viral plaques in culture.
you can buy it off the world wide web, or any chemical store actually.