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I assume this is a question about viewing an object through a compound microscope. Always use the shortest objective lens (that is the one with the lowest magnification) first. Focus the lens and then when you want more detail or a closer view, you can simply rotate the lens turret to a longer lens and it should be nearly in focus. Use the fine focus feature to sharpen the picture. If an object is viewed in this way, the danger of scratching the high power objective by ramming it into the slide is lessened.

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14y ago
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9y ago

The objective that is used to get a general view of the object will be the one that allows for the largest view. Some common general objectives are PLN-PH for olympus microscopes.

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Q: Which objective should be use first in viewing an object?
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