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What is meant by streak dilution technique?
The streak plate technique is a method of diluting bacteria down suficiently so that the will grow as single colonies. The technique varies from individual to individual so much so that you can identify a researcher's plates much like their handwritting! The technique is somewhat more standardised in hospital labs and a printed out sheet is placed below the plate for the operative to follow as a guide. The technique is usually taught like this; 1) Flame your loop and aseptically take 1 loopful of culture and place it a 12 o'clock on your plate draw a straight line 5cm across the plate ending around 2.30o'clock. 2) Lift the loop and draw two more lines parallel the first about 0.5 cm distance below the first. 3) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines over lapping the first set. (your end at 5o'clock) 4) Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the second set. (you end at 6.30o'clock) 5)Flame your loop. Turn the plate slightly anticlockwise and draw another set of 3 lines overlapping the third set. (your end at 8o'clock) 6) Flame your loop this time instead of a set of lines start by overlapping the fourth set of lines and then draw a scribble into the middle of your plate using as much of the unused agar as possible. The technique is sort of a dilution becasue each time you flame your loop it is sterilised, when you then draw out some of the bacteria from your last set of lines and spread them over a much greater area.
What are Two kinds of flame?
the yellow/safety flame - thats the one that burns less the blue flame - burns THE ROARING FLAME - that one burns a lot and you can tell the difference from the blue flame because it makes a roaring sound
What type of a flame is best to use?
if you are talking about what flame to use on a Bunsen burner than the yellow flame is to make sure that everyone knows that there is a flame but the blue flame (less visible) is used as the hotter flame and the better one.
Is flame an abstract noun?
No, the noun flame is usually a concrete noun, a word for a thing that can be seen, touched, sometimes smelled, and even measured for size and temperature; a flame is a physical thing. The noun flame is sometimes used in an abstract context, for example to refer to an 'old flame', or the 'flame of desire'. The word flame is also a verb (flame, flames, flaming, flamed).
How do you increase the temperature of a yellow flame coming out of a busen burner?
You can't it is always the same. You only use the blue flame to heat things because the yellow flame is the safety flame and the blue flame is hotter.
What is the purpose of the Bunsen burner in maintaining aseptic technique?
The purpose of the Bunsen burner in maintaining aseptic technique is to sterilize equipment and create a sterile work environment by providing a flame that can be used to heat and sterilize tools used in experiments, preventing contamination from microorganisms.
What is the purpose of flaming in the aseptic technique?
The purpose of flaming is to sterilize the surface of tools or equipment, such as inoculating loops or wire handles, in the aseptic technique. By passing the tool through a flame, it helps to kill any potential microorganisms present, reducing the risk of contamination during microbiological procedures.
Why we use blue flame to have an aseptic area?
A blue flame is used to create an aseptic area primarily because it indicates complete combustion, producing a hotter and cleaner flame compared to a yellow flame. This high temperature helps to sterilize surfaces and equipment by effectively killing bacteria and other microorganisms. Additionally, the blue flame minimizes the production of soot and harmful byproducts, creating a safer and more controlled environment for sterile work.
Why do we need to a Bunsen burner in aseptic techniques?
A Bunsen burner is used in aseptic techniques to create a sterile environment by providing a source of heat for sterilizing tools, such as inoculating loops and needles. The flame from the Bunsen burner can be used to sterilize the equipment before and after use to prevent contamination during experiments or procedures.
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
What do you call the technique when the flame comes out when the chef is cooking?
flambe..
What are the steps to preparing a smear aseptic?
To prepare an aseptic smear, first, gather your materials, including a clean glass slide and a sterile inoculating loop. Flame the loop until it’s red hot to sterilize it, then allow it to cool briefly before dipping it into the microbial culture. Gently spread a thin film of the culture on the slide, then air-dry it completely before fixing it by passing it through a flame. This process ensures that the sample is prepared in a sterile manner to avoid contamination.
What is smear preparation?
A smear preparation is a techniques that is used as a precursor is many different staining techniques.The smear prep usually calls for the sample bacterium to be placed on a microscope slide (use aseptic technique) with one drop of DI water. Then the slide is allowed to air dry for a few minutes then the slide is passed through a Bunsen burner flame. Use a cloths pin so that you do not burn you hands Only pass the glass microscope slide through the flame not you cloths pin if it is wood it will catch fire. Pass the slide through 3-4 times then you are ready to continue on with your desired staining technique
What is sterile technique what dose this involve when should this be used?
Sterile technique is a procedure that includes the medical and the laboratory techniques like with cultures. Sterile technique includes techniques such as flame sterilization and it should be used in hospital operating theatres.
What is the color of ethanol flame during combustion?
Etanol is burning with a faint blue-ish flame color
Who invented the flame test?
The flame test was not invented by a single person, but it has been used for centuries to identify elements based on the unique colors they emit when heated in a flame. The technique has been attributed to various scientists and chemists throughout history.
What ion gives a bright yellow-orange flame test?
Sodium ions give a bright yellow flame color during a flame test.
