To examine it under the microscope, the specimen needs to be illuminated by either a light underneath or a stream of electrons. If the specimen is too thick, and light or electrons cannot penetrate it, the scientist will be unable to see any detail.
Slide.
The course adjustment knob on a compound light microscope is used for making large changes in focus when initially locating a specimen. It is typically used with low-power objectives to quickly bring the specimen into view. Once the specimen is roughly focused, the fine adjustment knob should be used for precise focusing. Using the course knob with high-power objectives can risk damaging the slide or the lens.
start by focusing with the lowest lens to make sure the slide is right and you can see the specimen them proceed to the next lens power and focus again just to make the view clear then proceed to the highest and focus one more time
Wet mount.
A cover slip or cover glass is a thin flat piece of transparent material, usually square or rectangular, about 20 mm (4/5 in) wide and a fraction of a millimetre thick, that is placed over objects for viewing with a microscope. The object is usually held between the cover slip and a somewhat thicker microscope slide, which rests on the microscope's stage or slide holder and provides the physical support for the object and slip.The main function of the cover slip is to keep solid specimens pressed flat, and liquid samples shaped into a flat layer of even thickness. This is necessary because high-resolution microscopes have a very narrow region within which they focus.The cover glass often has several other functions. It holds the specimen in place (either by the weight of the cover slip or, in the case of a wet mount, by surface tension) and protects the specimen from dust and accidental contact. It protects the microscope's objective lens from contacting the specimen and vice-versa; in oil immersion microscopy or water immersion microscopy the cover slip prevents contact between the immersion liquid and the specimen. The cover slip can be glued to the slide so as to seal off the specimen, retarding dehydration and oxidation of the specimen. Microbial and cell cultures can be grown directly on the cover slip before it is placed on the slide, and specimens may be permanently mounted on the slip instead of on the slide.
the five steps to making a wet mount slide is 1.use a flat glass slide to prepare a wt mount slide 2.suck up a few drops of water from your liquid specimen into a medicaine dropper 3.pick up the wet mount slide on the 2 outer sides of the slides 4.place the specimen your using into the water 5.place the top cover slips on the top of the water with the specimen in it (: (: (: (: (: (: (: (: (: (:
The specimen should be placed on the top surface of the microscope slide. This allows the light to pass through the specimen from below and be magnified by the lenses in the microscope to form an image for observation.
A stained specimen slide typically requires more light than an unstained live specimen slide. This is because the staining process can reduce the transparency of the specimen, making it harder for light to pass through and creating a need for more illumination to visualize details.
A prepared slide is sealed by acrylic or any other medium. After the solvent evaporates, the specimen is locked under the slip. On the other hand, a wet mount slide has the specimen prepared just before it is put under microscope.
The small glass square is called a coverslip. It is used to place over the specimen and mounting medium on a wet mount slide to prevent evaporation, protect the specimen, and provide a flat viewing surface for microscopy.
To prepare a wet mount slide, place a small drop of liquid or specimen in the center of a clean microscope slide. Gently lower a coverslip onto the drop at a 45-degree angle to avoid trapping air bubbles. View the specimen under a microscope on low power to observe.
A wet mount slide is not recommended to be saved for a later class period as it can dry out and affect the quality of the specimen. It is best to prepare a new wet mount slide each time you want to examine a specimen to ensure accurate results.
You need the bottom of the the slide, the you put a specimen on it. After put a drop of water on it then put the cover and apply pressure.
A wet mount slide is made by placing the specimen in a drop of water on the slide and covering it with a glass slip. This technique is commonly used in microscopy to observe living organisms or specimens in their natural hydrated state.
To remove extra water from a wet mount slide, you can gently touch a piece of absorbent paper or tissue to the edge of the cover slip. Capillary action will draw the excess water towards the paper, helping to dry the slide. Be careful not to press too hard or you might disturb the specimen on the slide.
A slide that includes a specimen that has been stained and glued to it is typically referred to as a "permanent slide" or "mounted slide." These slides are prepared for long-term storage and observation, allowing for detailed examination of the specimen's structure and features under a microscope. The staining enhances contrast, making cellular components more visible, while the glue or mounting medium preserves the specimen.
A temporary mount is created by placing a specimen on a slide without using any mounting medium, while a permanent slide involves using a mounting medium (such as Canada balsam) to affix the specimen to the slide permanently. Permanent slides are more durable and suitable for long-term storage or examination, while temporary mounts are often used for quick observations or educational purposes.