Urea
Triglycerides hydrolysis test because this bacterium feeds on fatty acids.
it is positive for starch hydrolysis
Sucrose would not give a positive test with Fehling's reagent after hydrolysis because sucrose is a non-reducing sugar. During hydrolysis, sucrose is broken down into its monosaccharide components (glucose and fructose), which are reducing sugars and can react with Fehling's reagent to give a positive test for reducing sugars.
Bright pinkish-red.
Bacillus cereus is positive for gelatin hydrolysis. This bacteria is capable of hydrolyzing gelatin(a protein), thus, a nutrient gelatin medium inoculated with this bacteria will remain liquid when placed in an ice bath (don't agitate).the bacteria had proteases that is responsible for the hydrolysis of the gelatin..
A positive test for gelatin hydrolysis is indicated by the liquefaction of gelatin media after incubation. This occurs when gelatinase, an enzyme produced by certain bacteria, breaks down gelatin into its soluble components. As a result, the initially solid gelatin medium becomes liquid, demonstrating that the organism can hydrolyze gelatin. The presence of a clear zone around colonies in the medium is also indicative of positive hydrolysis.
Yes
Shigella dysenteriae is generally considered negative for starch hydrolysis. In laboratory tests, it does not produce the enzyme amylase, which is required to hydrolyze starch into simpler sugars. Therefore, when inoculated on starch agar, it typically does not show a clear zone of hydrolysis around the colonies.
It is asset sensitive
A positive test for casein hydrolysis is indicated by a clear zone around the growth of the bacteria on a casein-containing medium, such as milk agar. This clear zone signifies that the bacteria have produced caseinase, an enzyme that breaks down casein into smaller peptides and amino acids, thereby reducing the opacity of the medium. If the medium remains opaque, it indicates that casein hydrolysis has not occurred.
The indicator used to test for protein hydrolysis that results in a yellow color is phenol red. In an alkaline environment due to the release of ammonia from protein breakdown, phenol red changes from red to yellow, indicating a positive test for protein hydrolysis.
Cation hydrolysis involves the reaction of a cation with water to form a hydroxide ion and an acidic solution, whereas anion hydrolysis involves the reaction of an anion with water to form a hydroxide ion and a basic solution. Cation hydrolysis leads to acidic conditions due to the release of H+ ions, while anion hydrolysis results in basic conditions due to the release of OH- ions.