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To make platelet diluting fluid, you typically combine a specific volume of sodium chloride (NaCl) with a buffer solution, often containing sodium citrate or a similar anticoagulant to prevent platelet aggregation. The common formulation includes 0.85% NaCl in a phosphate-buffered saline (PBS) solution. Adjust the pH to around 7.4 and ensure it is sterile before use. Always follow specific protocols or guidelines provided for the intended application.
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What diluting means?
To mix a fluid or chemical with water to make it not as strong.I love bunnies!
Name of RBC diluting fluid?
Gower's solution
Why wbc diluting fluid hemolyze rbc?
The distilled water present in the WBC diluting fluid is not isotonic and therefore is responsible for the destruction of RBCs. DR. Umesh Ghate and Shruti Salian
What is the chemical composition of RBC diluting fluid?
RBC diluting fluid commonly consists of an isotonic saline solution containing electrolytes and buffers to maintain the stability of red blood cells during counting. The composition may vary based on the specific laboratory protocol or commercial product used.
What is the role of RBC diluting fluid?
The role of RBC diluting fluid in laboratory testing is to dilute blood samples to a standardized concentration for accurate cell counting and analysis. It helps ensure that the sample is within the measurable range of the instrument, enabling accurate and reproducible results.
Does diluting acids in water make them weaker?
Yes
Use of sodium citrate in RBC diluting fluid?
Sodium citrate is used in RBC diluting fluid to prevent clotting by chelating calcium ions, which are necessary for clot formation. It helps to maintain the integrity of the red blood cells and allows for accurate cell counting and sizing in hematological analysis.
What is it called when you make an acid weaker by mixing it with water?
diluting
Why to use RBC diluting fluid for counting?
You can use any isotonic solution like Normal Saline Solution (NSS), Hayem's and Gower's.
Why the diluting fluid cause haemolysis of red blood cells but not the white blood cells in total leukocytes counting?
Glacial Acetic Acid
How do you dilute 0.1N to make 0.5N?
diluting 0.1 to prepare 0.5 is impossible
What is the function of gentian violet in wbc diluting fluid?
Gentian violet is used in white blood cell diluting fluid to help stain the nuclei of white blood cells, making them more visible for counting and identification under the microscope. This staining enhances the accuracy of the white blood cell count in a blood sample.
