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What is the chemical composition of RBC diluting fluid?
RBC diluting fluid commonly consists of an isotonic saline solution containing electrolytes and buffers to maintain the stability of red blood cells during counting. The composition may vary based on the specific laboratory protocol or commercial product used.
Why wbc diluting fluid hemolyze rbc?
The distilled water present in the WBC diluting fluid is not isotonic and therefore is responsible for the destruction of RBCs. DR. Umesh Ghate and Shruti Salian
What is the role of RBC diluting fluid?
The role of RBC diluting fluid in laboratory testing is to dilute blood samples to a standardized concentration for accurate cell counting and analysis. It helps ensure that the sample is within the measurable range of the instrument, enabling accurate and reproducible results.
Use of sodium citrate in RBC diluting fluid?
Sodium citrate is used in RBC diluting fluid to prevent clotting by chelating calcium ions, which are necessary for clot formation. It helps to maintain the integrity of the red blood cells and allows for accurate cell counting and sizing in hematological analysis.
Why to use RBC diluting fluid for counting?
You can use any isotonic solution like Normal Saline Solution (NSS), Hayem's and Gower's.
Why wbc diluting fluid lysis rbc but not rbc?
WBC diluting fluid is designed to lyse red blood cells (RBCs) while preserving white blood cells (WBCs) for counting and analysis. The lysis occurs due to the specific osmotic properties and chemical composition of the diluent, which affects the RBC membranes but not the more robust WBC membranes. This selective lysis allows for accurate WBC enumeration without interference from RBCs. The fluid typically contains agents that disrupt the lipid bilayer of RBCs, leading to their breakdown while maintaining the integrity of WBCs.
What are the functions of the various constituents of RBC diluting fluid?
sodium citrate prevents coagulation of blood and provide correct osmotic pressure. formalin acts as a preservative and checks bacterial and fungal growth.
What is the Difference between rbc thoma pipette and wbc thoma pipette?
The difference between the two is that the RBC Diluting pipette counts the erythrocytes (Red blood cells) while the other one counts the leukocytes (White blood cells) and that the pipette (RBC) itself has a more circular body compared to WBC diluting pipete. On the other hand, WBC diluting pipette has a pointed tip unlike the RBC Diluting pipette. -Fer Philippines <3
Why bulb of wbc smaller than rbc pipette?
Rbc pipette is bigger than the wbc pipette to dilute the blood with fluid.
What diluting means?
To mix a fluid or chemical with water to make it not as strong.I love bunnies!
Another name for erythrocytes?
RBC
How do you count the RBC and WBC counting?
counting of Rbc and Wbc is done throug hemocytometer .duilting fluid are needed to destory unwanted things which can confuse us in various ways .
