The insulin that we engineer bacteria to make (E.colidoes not naturally make insulin) is identical to human insulin.
Scientists have put some bacteria to work making medicines and other substances. The first medicine-producing bacteria were made in the 1970s. By manipulating the bacteria's genetic material, scientists engineered bacteria to produce human insulin. Although healthy people can make their own insulin, those with some types of diabetes cannot. Many people with diabetes need to take insulin daily. Thanks to bacteria's fast rate of reproduction, large number of insulin-making bacteria can be grown in huge vats. The human inlusin they produce is then perified and made into medicine. CREDITS FROM: NORTH CAROLINA 8TH GRADE PRENTICE HALL SCIENCE EXPLORER Pg.225
Well, the insulin a diabetic gets does not contain any bacteria. It is sterile. However, the insulin is made by bacteria, usually E. coli. Hope this helps! Some Stuff about the E.Coli Bacteria: • • The Bacteria E.coli used to produce insulin for those with diabetes • It's Inexpensive - compared with 1980 methods(extracting insulin from pigs etc.) • Large quantities of the modified bacteria are killed and insulin is extracted and purified. •The Bacteria E.coli used to produce insulin for those with diabetes
The process involves inserting the human gene for insulin into a bacterial plasmid, which acts as a vector. The bacteria then replicates the gene and produces insulin protein. The protein is harvested, purified, and formulated into insulin for medical use.
Human plasmids introduced into the bacteria stimulate insulin production. A special enzyme is used to cut out the insulin gene from a human cell. It is attached to a bacterial chromosome which is also split open by an enzyme. The gene is then transferred into a bacterial cell. The gene makes the bacterial cell produce insulin.
Insulin is usually made in your body automatically; diabetes is when your body does not make enough insulin. So some people with diabetes have to take insulin to control their blood sugar.
In 1983 scientists worked out a way of producing human insulin on a large scale using genetically modified bacteria. They did this by first working out which human chromosome was responsible for producing insulin. They then isolated that chromosome using a restricting enzyme. They would then remove the plasmid from the bacterium, cut out a small piece of the plasmid using enzymes and then replace the gap made with the human insulin gene. The genetically engineered plasmid is the inserted into a new bacterium and this bacterium is allowed to grow and multiply. Each of the new bacteria would contain the human insulin gene and as the bacteria continued to multiply they would be producing insulin which could then be used to treat diabetes. In this way we could produce large amounts of insulin that was suitable for vegetarian use and causes very few allergic reactions quickly and cost effectively.
It would be more correct to say that bacteria and yeast can be genetically modified to produce a compound which is chemically identical to human insulin.So can plants, by the way; a safflower plant with the genes to produce bio-identical human insulin has been demonstrated.
Insulin can be synthetically made through a process called recombinant DNA technology. This involves inserting the gene for human insulin into bacteria or yeast cells, which then produce insulin that is identical to what the human body makes naturally. These cells are then cultured and the insulin is harvested and purified for medical use.
Human insulin is preferable to animal insulin because it is made through genetic engineering, ensuring higher purity and reducing the risk of allergic reactions. Additionally, human insulin closely mimics the natural insulin produced by the human body, which leads to more predictable and consistent results in managing diabetes. Lastly, human insulin is now more widely available and cost-effective compared to animal-derived insulin.
genetic engineering techniques such as recombinant DNA technology, where the gene encoding for insulin production was introduced into the bacterial DNA. These bacteria then use this genetic information to produce insulin, which can be harvested for diabetic treatment.
Yes. In fact, currently insulin manufacturers worldwide use transgenic (recombinant) bacteria to produce insulin efficiently.