muscle biopsy
This process is used as a screening test for bacteria only.
A thin stained slice of tissue mounted on a microscope slide is called a histological slide. These slides are used for microscopic examination to study the cellular structure and organization of tissues.
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Microscopic examination of the tissue
Cells are stained during microscopic analysis to make them easier to see and distinguish under the microscope. Staining helps highlight different structures within the cell, making it possible to study their characteristics and functions more effectively.
Air-drying smears during bacterial staining is essential to fix the cells to the slide. This helps prevent cell loss when they are subsequently stained and washed in the staining process. Air-drying also helps to preserve the cellular morphology and structure for accurate microscopic examination.
Ascaris lumbricoides eggs are bile stained in stool examination because they contain a yellow-brown proteinaceous layer that is stained by bile salts. This characteristic staining helps in identifying the eggs under the microscope during laboratory analysis.
this type of microscopic examination's main purpose is to observe the motility of a microorganism however these organisms do eventually die. so when a wet prep is made it needs to be exmained immediately whereas a stained prep can be examined a little afterwards and can also be stored.
Parasite eggs are often bile stained due to their passage through the host's digestive system, particularly in the liver and intestines where bile is produced. Bile, which contains bile salts and pigments like bilirubin, can adhere to the eggs as they are excreted in feces. This staining can aid in the identification of certain parasite species during microscopic examination, as the color difference helps differentiate them from non-parasitic material.
Pulling the stained material apart with needles helps to reduce overlapping of tissue sections, allows for better visualization of individual cells or structures, and prevents bubbles from forming under the cover slip during mounting. This process enhances the clarity and quality of the microscopic image.
Paraffin examination is a process in pathology where tissue samples are embedded in paraffin wax, sliced thinly, stained, and examined under a microscope. This method allows for detailed analysis of tissue structure and identification of abnormalities or diseases within the sample. Paraffin sections are commonly used for diagnosing conditions like cancer.
The essence of making a thin smear is to spread a sample of cells, bacteria, or other substances onto a slide in a very thin layer. This allows for microscopic examination of the sample, so that individual cells and components can be observed. Making a thin smear involves taking a small sample of the material, suspending it in a liquid, and using a microscope slide to spread the sample evenly across the slide. The sample must be spread thinly enough so that individual cells are visible. When the slide is stained, the thin smear allows individual cells to be identified and counted. Making a thin smear is a fundamental part of many laboratory procedures, such as microscopic examination of blood or body fluids, or bacterial culturing.