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Shigela flexneri or shigella sonnei
To differentiate between Salmonella enteritidis and Shigella sonnei, the triple sugar iron (TSI) agar test can be used. Salmonella enteritidis typically produces hydrogen sulfide (H2S), resulting in black colonies on TSI agar, while Shigella sonnei does not produce H2S and will not change the color of the agar. Additionally, biochemical tests such as urease and indole tests can further help distinguish between the two, as Shigella sonnei is urease negative and indole positive, while Salmonella enteritidis is urease negative and indole negative.
S. aureus, E. coli, P. auriginosa, Salmonella typhi, Shigella sonnei, Shigella flexneri, Vibrio cholera.
Shigella is caused by a type of bacterium. Specifically, it is a genus of bacteria belonging to the family Enterobacteriaceae. Shigella is known to cause shigellosis, an infectious disease characterized by diarrhea, fever, and abdominal cramps. There are four main species of Shigella, with Shigella dysenteriae, Shigella flexneri, Shigella boydii, and Shigella sonnei being the most common.
Salmonella, Shigella Organisms that ferment lactose display "nucleated colonies" - colonies with dark centers.
S.sonnei is not a true lactose fermenting bacteria (see.. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=271578&blobtype=pdf)But is a delayed lactose fermenter as it does not have the enzyme permease to transport lactose through its cell wall.However an ONPG lactose fermenting test does not require permease to allow the bacterium to ferment lactose so in an ONPG test the shigella sonnei will appear as lactose fermenting positive.Hope this helps!!2nd year BMS student
S. dysenteriae generally produces the most severe attacks, and S. sonnei the mildest attacks of shigellosis.
Its currently an obsolete term. Previously used to denote the late lactose fermentors like Shigella sonnei, citrobacter etc.
i did tests on shigella dysenteriae the tube was purple top yellow bottome for the decarboxylase and purple top and bottom with a yellow mid for the LIA control meaning that the organism can utilise lysine as the tube has gone purple at the bottom
Mnemonic: KEE Klebsiella pneumoniae Enterobacter cloacae E. coli Shigella sonnei can ferment lactose, but only after prolonged incubation and so it is referred to as a '''''late-lactose fermenter'''''. Vibrio cholerae is also a late lactose fermenter.
To differentiate Shigella flexneri from Yersinia pseudotuberculosis, a key lab test is the urease test. Yersinia pseudotuberculosis is urease positive, while Shigella flexneri is urease negative. Additionally, biochemical tests such as the fermentation of lactose can help, as Shigella flexneri does not ferment lactose, whereas Yersinia may show variable lactose fermentation.