many gram positive bacilli
Gram Negative.
A negative result for a spore stain indicates that the organism does not form endospores. Endospore formation is a survival mechanism for some bacteria to withstand harsh environments, so a negative result suggests that the organism may be more susceptible to environmental stressors.
Human cells have no cell walls, only a plasma membrane, and as a result lack the peptidoglycan layer that gram stains utilize to differentiate between gram negative and gram positive species of bacteria. Therefore, human cells are unable to retain the crystal violet introduced in the first step of the gram stain, and stain negative. (they appear pinkish like gram-negative bacteria)
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Gram stain
Gram Negative
Protists are often stained using a silver stain, not a Gram stain.
The Gram stain is used for bacteria and not for viruses.
Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.
The counter or secondary stain used in the Gram stain procedure is safranin.