Gower's solution
The distilled water present in the WBC diluting fluid is not isotonic and therefore is responsible for the destruction of RBCs. DR. Umesh Ghate and Shruti Salian
To perform an erythrocyte count, a diluting fluid is used to prepare the blood sample for accurate counting under a microscope. Commonly used diluting fluids include saline or a buffered solution, such as Hayem's solution, which contains sodium chloride and formaldehyde to preserve the cells. The dilution factor typically ranges from 1:200 to 1:400, depending on the specific protocol, to ensure that red blood cells are adequately separated for counting. Proper dilution is crucial for obtaining reliable and reproducible results in hemocytometry.
RBC diluting fluid commonly consists of an isotonic saline solution containing electrolytes and buffers to maintain the stability of red blood cells during counting. The composition may vary based on the specific laboratory protocol or commercial product used.
To mix a fluid or chemical with water to make it not as strong.I love bunnies!
The role of RBC diluting fluid in laboratory testing is to dilute blood samples to a standardized concentration for accurate cell counting and analysis. It helps ensure that the sample is within the measurable range of the instrument, enabling accurate and reproducible results.
Sodium citrate is used in RBC diluting fluid to prevent clotting by chelating calcium ions, which are necessary for clot formation. It helps to maintain the integrity of the red blood cells and allows for accurate cell counting and sizing in hematological analysis.
red blood cells are also called erythrocytes
Glacial Acetic Acid
You can use any isotonic solution like Normal Saline Solution (NSS), Hayem's and Gower's.
Gentian violet is used in white blood cell diluting fluid to help stain the nuclei of white blood cells, making them more visible for counting and identification under the microscope. This staining enhances the accuracy of the white blood cell count in a blood sample.
The mean corpuscular volume is the measure that determines the average size of an erythrocyte. The erythrocyte is what is commonly known as red blood cells.