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Laboratory diagnosis of stachybotryotoxicosis primarily involves the identification of Stachybotrys chartarum, the fungus associated with the condition, in environmental samples such as mold or dust. This can be achieved through culture methods, PCR assays, or microscopic examination. Additionally, biomarkers such as specific mycotoxins produced by the fungus may be detected in biological samples, including urine or blood, to support the diagnosis. Clinical evaluation of symptoms and exposure history are also essential components of the diagnostic process.

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