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The Ames assay is a pre-clinical testing assay that uses bacteria to determine if a compound causes genetic damage. It is used by a variety of companies (pharmaceutical, cosmetic, petroleum) to assess whether or not new compounds cause DNA damage and thus, potentially, cancer.
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What is the purpose of the ames test?
The Ames test identifies chemical mutagens.
Can you reduce animal killing using AMES test?
not with ames but with animes
Are substances that test positive with the Ames test necessarily carciongenic in humans?
The substances that are test positive with Ames test may or may not be carcinogenic for humans. Similarly, some substances that cause cancer in laboratory animals do not give a positive result Ames test. It is not possible to decide the carcinogenicity of any substances only depending on the Ames test.
What is the principle behind the kraut's test for lipids?
The principle behind the Kraut's test for lipids is that lipids form a white emulsion when shaken with sulfuric acid due to the formation of glycerol and fatty acids. The appearance of a white emulsion indicates the presence of lipids in the test sample.
In what field of science would one carry out an Ames test?
Ames test would typically be carried out in the field of biology, more specifically toxicology. The Ames test involves testing genetics, which involves taking a sample and examining it closely in a laboratory.
What is the test used for detecting chemicals with carcinogenic potential?
The test used for detecting chemicals with carcinogenic potential is the Ames test. This test uses bacteria to detect mutations induced by the chemical being tested, which can indicate its carcinogenic potential.
What is the principle behind alkaloidal test?
The principle behind alkaloidal tests is based on the ability of alkaloids to form specific chemical reactions or complexes when exposed to certain reagents. These reactions can help in identifying the presence of alkaloids in a given sample by producing characteristic color changes or precipitation. Common alkaloidal tests include Dragendorff's test, Mayer's test, and Wagner's test.
Describe the principle of the Ames test for identifying chemical carcinogens?
The Ames test is a widely used assay that assesses the mutagenic potential of chemicals by monitoring their ability to induce mutations in Salmonella bacteria. If a chemical is mutagenic, it can increase the rate of mutation in the bacteria, which can lead to cancer development in humans. By comparing the mutation rate of bacteria exposed to a test chemical with a control group, researchers can identify potentially carcinogenic compounds.
What is the principle behind the shinoda test for flavonoids?
In the Shinoda test for flavonoids, a sample is added to pieces of magnesium and then hydrochloric acid is dripped on the sample. The color the sample turns indicates if there are flavonoids present or not.
Purpose of liver extract in the Ames test?
Ames test is used to test whether a certain substance is mutagenic. Liver extract is optionally added to simulate the effect of metabolism, as some compounds, like benzo[a]pyrene, are not mutagenic themselves but their metabolic products are.
Principle of widal test?
same principle for latex methd
What is the principle behind the use of the refractometer in determining salinity?
it works on the principle of refraction
