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In hemoglobin, the ratio of heme to globin is 1:1, meaning each hemoglobin molecule contains one heme group and one globin chain. Hemoglobin is composed of four globin chains (two alpha and two beta chains in adults) and four heme groups, resulting in a total of four heme groups per hemoglobin molecule. Therefore, while the individual heme to globin ratio is 1:1, the overall structure includes a total of four heme groups associated with the four globin chains.

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