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What is the different between polyclonal antibody and antiserum?
polyclonal antobody is the antibody produced for many or non specific antigens but antiserum is the antibody for a specific antigen
What is the basic principle of the antigen-antibody reaction?
-the principle of antigen-antibody reaction is a process of the immune system in which immunoglobulin-coated B cells recognize a specific antigen and stimulate antibody production. T cells also play an essential role in the reaction. An antigen-antibody reaction begins with the binding of antigens to antibodies to form antigen-antibody complexes. These complexes may render toxic antigens harmless (neutralization), agglutinize antigens on the surface of microorganisms, or activate the complement system by exposing the complement binding sites on antibodies. Certain complement protein molecules immediately bind to these sites and trigger the activity of the other complement protein molecules, which cause antigen-bearing cells to lyse. Antigen-antibody reactions may start immediately with antigen contact or as much as 48 hours later. They normally produce immunity but may also be responsible for allergy, autoimmunity, and fetomaternal hematologic incompatibility. In the immediate allergic response, the antigen-antibody reaction activates certain enzymes and causes an imbalance between those enzymes and their inhibitors
A reaction between an antibody and soluble antigen-forming lattices is called?
precipitation reaction
What is the difference between alloimmunization and isoimmunization?
the autoimmune reaction is the reaction between the antibody and antigene when both come from the same body but the isoimmune reaction is between the antigene and antibody produced for the same antigene ( the same according to the type but not the immunity )
What is the difference between agglutinogens and agglutinin?
When the immune system cause cross linking of cells or particles an agglutination reaction occurs and the responsible antibody is an agglutininAgglutinogens are1. any substance that, acting as an antigen, stimulates the production of agglutinin.2. the particulate antigen used in conducting agglutination tests.
What is a difference between passive and active agglutination?
The only difference between the two is with active agglutination you have a particulate Ag + Ab, since the Ag is particulate, large, when a complex is formed it is visable. In passive agglutination the Ag is soluble so it must first be attached to something like latex beeds or a carrier so when agglutination occurs it can be seen with the naked eye.
How to determinaion the A2B blood group?
First off test your blood with antibody's against A and B. The lab rats do this by making a solution of your blood and mixing this in several tubes with the antibody's. In your case both A and B will become clotted, because the antibody's against A react with your A antigens, and the antibody's against B react with your B antigens on the red bloodcells. But, if you really have A2, the reaction between the Anti-A and A will be slightly less powerfull. To determine whether you have A2, the blood solution is mixed with antibodies against A1. If this doens't become react and become clotted, you don't have A1, thus you have A2 (there are other possibilities but the lab always gives it the name A2). And there you go, now the lab rats know you have the A2B bloodtype. Ruben
Contrast between agglutinin and agglutinogen?
When the immune system cause cross linking of cells or particles an agglutination reaction occurs and the responsible antibody is an agglutininAgglutinogens are1. any substance that, acting as an antigen, stimulates the production of agglutinin.2. the particulate antigen used in conducting agglutination tests.
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
The secondary antibody in an ELISA test is conjugated with an enzyme to amplify the signal produced when the antibody binds to the target antigen. This enzyme-substrate reaction generates a detectable signal that indicates the presence of the antigen, which allows for more sensitive and accurate detection in the ELISA assay.
Are the genes coding for one antibody entirely different from those coding for a different antibody?
no, not entirely. Each antibody (Immunoglobulin, "Ig") has both 2 constant regions referred to as "heavy hains" and 2 variable regions referred to as "light chains". The structure of the heavy chains remain the same between the different antibody structures. However, the light chains differ in structure enabling them to respond differently to different specific antigens introduced to the cellular membrane.
What is the difference between antibodies and antigens?
Antigen is a substance that can induce the generation of antibodies, any substance that can induce immune response. Antibody is a protective protein produced by the body in response to an antigen.
Are chickenpox antibody and shingles antibody the same?
Chickenpox and shingles result from the same virus, and generate the same antibodies. There is no difference between chickenpox antibody and shingles antibody, and there is only one test (varicella virus antibody) for both.
