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For the contraction of the sarcomere to occur, calcium ions (Ca²⁺) must be present. When a muscle cell is stimulated, calcium is released from the sarcoplasmic reticulum, binding to troponin on the actin filaments. This interaction causes a change in the conformation of tropomyosin, exposing binding sites for myosin, allowing the myosin heads to attach and pull the actin filaments, resulting in contraction.

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