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E. coli remains pink during gram staining because it is a Gram-negative bacterium, which has a thin peptidoglycan layer and an outer membrane that does not retain the crystal violet stain, leading to coloration by the counterstain (safranin). Similarly, B. subtilis, although a Gram-positive bacterium, can appear pink if improperly stained or if the peptidoglycan layer is damaged, allowing it to lose the crystal violet and take up the counterstain. Proper techniques and timing during the staining process are crucial for accurate identification.
What else can I help you with?
WHAT IS THE COLOUR OF BACILLI SUBTILIS IN MacConkey agar?
Bacillus subtilis typically does not grow on MacConkey agar, as it is a selective medium for gram-negative bacteria. However, if it does grow, it would appear as pale or colorless colonies because B. subtilis is a gram-positive bacterium and does not ferment lactose, which is what the agar is designed to detect.
What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Why do you look at cultures that had been grown for different lengths of time during endospore staining?
You would look at cultures that had been grown for different lengths of time during endospore staining to ensure that the cells had been agitated enough to soak up the dye. This is done at 12, 24, and 36 hours.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
Without heat fixing, the bacteria on the slide will not adhere properly, leading to poor attachment to the slide during staining. This may result in uneven staining, leading to difficulty in visualizing the bacterial cells clearly under the microscope. Proper heat fixing ensures that the bacteria are securely attached to the slide, allowing for better staining and clearer observation under the microscope.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
Factor that would give False results in gram staining?
Several factors can lead to false results in Gram staining, including improper sample handling or storage, which may cause bacterial cell degradation. Inadequate fixation of the sample can result in the loss of bacteria during staining. Additionally, over-decolorization or under-decolorization can misclassify Gram-positive bacteria as Gram-negative or vice versa. Lastly, using old or expired reagents can also affect the staining outcome.
When staining ecoli with methylene blue what would you see?
blue bacilli
What is made by staining and arrganging chromosomes so that they can be viewed microscopically?
A karyotype is made by staining and arranging chromosomes so that they can be viewed microscopically.
Brown staining on teeth following wisdom teeth extraction?
More than likely, that is just dried blood. Nothing else would cause staining in removal of wisdom teeth.
What leukocyte is formed in bone marrow and having neutral staining granules?
That would be a Neutrophil. :)
When tea staining paper do you tea stain or write first?
write on it first, just make sure when your tea staining it the teabag you're using isn't soaking wet, as this would smudge the ink
What statement would a critic of capitalism have most likely made during the industrial revolution?
The predominant criticism would be that business owners get rich while workers remain poor.
