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What are the results for Pseudomonas aeruginosa in phenol red broth?
Negative for both. Pseudomonas does not ferment sugars.
Does phenol give a positive biuret test?
No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.
What is the result of iodoform reacting with phenol?
When iodoform reacts with phenol, the iodoform is reduced to triiodomethane and phenol is oxidized to benzoquinone. The reaction results in the formation of a white precipitate of triiodomethane.
Why are xanthoproteic and millon-nasse test unsatisfactory for the use in examination for proteins?
The xanthoproteic test involves nitration of aromatic amino acids in proteins, which may not always be specific for proteins as other compounds containing these amino acids can give false positive results. The Millon-Nasse test is based on the reaction of phenol groups in proteins, which can also react with other substances containing phenol groups, leading to false positive results. Overall, these tests lack specificity and can give inaccurate results when used to examine proteins.
Why normal ferric chloride is not used in detection of phenol?
Normal ferric chloride gives a positive test for phenol as it forms a violet complex with it. This can lead to false positives in other compounds that also react with ferric chloride, making it unreliable for detecting phenol specifically. Instead, a modified version called FeCl3/KI reagent is commonly used for phenol detection as it gives a distinct green color with phenol, allowing for more accurate and specific results.
What does the positive KMnO4 test indicate?
It means that it tests positive for either aldehyde, alkene, or phenol functional groups.
Used to test for protein hydrolysis which was indicated by a yellow color?
The indicator used to test for protein hydrolysis that results in a yellow color is phenol red. In an alkaline environment due to the release of ammonia from protein breakdown, phenol red changes from red to yellow, indicating a positive test for protein hydrolysis.
Explain the purpose of the phenol red in the medium?
To determine if a bacterium can ferment a particular carbohydrate and determine the end products of that fermentation
Why do ibuprofen and naproxen not give a positive test with 1 percent ironIII chloride?
Iron (III) Chloride reacts with the Phenol group. Ibuprofen and Naproxen do not have a phenol group. Acetaminophen, on the other hand, does have a phenol group with would indicate a positive test (purple color change) with in the presence of Iron (III) Chloride.
Why isn't phenol miscible in tris buffe?
Phenol is not miscible in tris buffer because phenol is a hydrophobic compound, while tris buffer is an aqueous solution. Hydrophobic compounds like phenol tend to separate from water-based solutions like tris buffer due to differences in polarity and interactions with water molecules. This results in the immiscibility of phenol in tris buffer.
What is the result when a Lucas test is done on a Phenol?
A positive Lucas test on phenol would indicate that phenol is a strong enough acid to react with Lucas reagent (a mixture of concentrated hydrochloric acid and zinc chloride) to form a cloudy solution or a precipitate. This reaction distinguishes phenol from other alcohols that do not react with the Lucas reagent.
Explain the function of phenol red in the urea broth medium?
phenol red act as the pH indicator. As the substrate urea is split into products, the presence of ammonia caused alkaline environment that cause the phenol red to turn to deep pink. Useful to test Proteus vulgaris.
