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Why are xanthoproteic and millon-nasse test unsatisfactory for the use in examination for proteins?
The xanthoproteic test involves nitration of aromatic amino acids in proteins, which may not always be specific for proteins as other compounds containing these amino acids can give false positive results. The Millon-Nasse test is based on the reaction of phenol groups in proteins, which can also react with other substances containing phenol groups, leading to false positive results. Overall, these tests lack specificity and can give inaccurate results when used to examine proteins.
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What are the principles involved in xanthoproteic test?
The xanthoproteic test is a test for the detection of proteins. If proteins are present, concentrated nitric acid reacts with the proteins to form a yellow color that turns orange-yellow by the addition of alkali. This is called the xanthoproteic reaction.
Is albumin positive to xanthoproteic test?
Yes, albumin is positive to the xanthoproteic test. The xanthoproteic test is used to detect the presence of proteins containing aromatic amino acids, such as albumin, by forming a yellow color when treated with nitric acid.
Do albumin gives a positive xanthoproteic test?
I reckon the answer would be that phenol has a hydroxyl group (-OH) which is bonded to a phenyl ring. It yields the same positive result like tyrosine which has a hydroxyl group bonded to its phenyl ring.
Do you expect adrenaline to give a positive xanthoproteic test?
No, adrenaline is not expected to give a positive xanthoproteic test. The xanthoproteic test is used to detect the presence of proteins containing aromatic amino acids, which react with nitric acid to form a yellow-colored compound. While adrenaline does contain an aromatic ring, it is classified as a catecholamine, not a protein, and therefore does not typically produce a positive result in this test.
What samples did not give a positive test with xanthoproteic test why?
The xanthoproteic test is used to detect the presence of aromatic amino acids, particularly tyrosine and tryptophan, by turning yellow upon reaction with nitric acid. Samples that do not contain these amino acids, such as certain non-aromatic amino acids (e.g., glycine, alanine) or peptides and proteins lacking these residues, will not yield a positive test. Additionally, any substances that do not contain proteins or peptides, such as carbohydrates or lipids, will also not give a positive result. Thus, the absence of aromatic amino acids or proteins in the sample is the reason for a negative xanthoproteic test.
Will adrenaline give a positive xanthoproteic test?
No, adrenaline will not give a positive xanthoproteic test. The xanthoproteic test is used to detect the presence of proteins, not hormones like adrenaline. Adrenaline is a hormone that is involved in the body's fight-or-flight response, and it does not react in the same way as proteins do in the xanthoproteic test.
What are the principles involved in xanthoproteic test?
The xanthoproteic test is a test for the detection of proteins. If proteins are present, concentrated nitric acid reacts with the proteins to form a yellow color that turns orange-yellow by the addition of alkali. This is called the xanthoproteic reaction.
Is albumin positive to xanthoproteic test?
Yes, albumin is positive to the xanthoproteic test. The xanthoproteic test is used to detect the presence of proteins containing aromatic amino acids, such as albumin, by forming a yellow color when treated with nitric acid.
How xanthoproteic acid affect on protein?
Xanthoproteic acid is used to detect the presence of proteins by forming a yellow color when it reacts with proteins that contain aromatic amino acids, such as phenylalanine, tyrosine, and tryptophan. This reaction helps in identifying proteins in laboratory settings by providing a colorimetric test for protein presence.
Do albumin gives a positive xanthoproteic test?
I reckon the answer would be that phenol has a hydroxyl group (-OH) which is bonded to a phenyl ring. It yields the same positive result like tyrosine which has a hydroxyl group bonded to its phenyl ring.
Do you expect adrenaline to give a positive xanthoproteic test?
No, adrenaline is not expected to give a positive xanthoproteic test. The xanthoproteic test is used to detect the presence of proteins containing aromatic amino acids, which react with nitric acid to form a yellow-colored compound. While adrenaline does contain an aromatic ring, it is classified as a catecholamine, not a protein, and therefore does not typically produce a positive result in this test.
What samples did not give a positive test with xanthoproteic test why?
The xanthoproteic test is used to detect the presence of aromatic amino acids, particularly tyrosine and tryptophan, by turning yellow upon reaction with nitric acid. Samples that do not contain these amino acids, such as certain non-aromatic amino acids (e.g., glycine, alanine) or peptides and proteins lacking these residues, will not yield a positive test. Additionally, any substances that do not contain proteins or peptides, such as carbohydrates or lipids, will also not give a positive result. Thus, the absence of aromatic amino acids or proteins in the sample is the reason for a negative xanthoproteic test.
What is the chemical equation of xanthoproteic test?
There is no set chemical formula for xanthroproteic acid. It is a yellow nitrophenyl complex that is derived from proteins when they are treated with nitric acid.
What is the basis for the xanthoproteic and biuret tests?
The xanthoproteic test is based on the reaction between phenolic amino acids, such as tyrosine, and concentrated nitric acid, which produces yellow-colored nitrophenolic compounds, indicating the presence of proteins. The biuret test, on the other hand, relies on the formation of a violet complex when proteins containing peptide bonds react with copper sulfate in an alkaline solution. Both tests are used to detect and confirm the presence of proteins in a sample.
Does adrenaline give a positive result in xanthoproteic?
Yes, adrenaline can give a positive result in the xanthoproteic test. This test identifies proteins containing aromatic amino acids, such as phenylalanine and tyrosine, which are present in adrenaline. When the adrenaline is heated with concentrated nitric acid, it forms a yellow-colored compound, indicating a positive result.
Why does concentrated HNO3 stain skin yellow?
Concentrated HNO3 (nitric acid) can react with the proteins in the skin to form a yellow-colored compound called xanthoproteic acid. This compound can absorb light in the visible spectrum, resulting in a yellow stain on the skin. This reaction is a chemical burn and should be treated immediately.
What functional group gives a positive test in the xanthoproteic test?
The phenolic functional group gives a positive test in the xanthoproteic test. This test involves the reaction of phenolic compounds with concentrated nitric acid, resulting in a yellow coloration due to the formation of nitrophenols.
