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False negatives or positives in ion testing can result from various factors, including sample contamination, improper handling, or inadequate testing methods. For instance, interfering substances in the sample may mask the presence of certain ions, leading to a false negative, while high concentrations of other ions can trigger a false positive. Additionally, variations in pH or temperature can affect the assay's accuracy. Lastly, human error during sample collection or analysis can also contribute to inaccurate results.

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