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Bial's test is a chemical test used to detect the presence of pentoses, a type of sugar. It involves the reaction of pentoses with orcinol in the presence of hydrochloric acid, producing a characteristic green or blue color. This test is particularly useful in differentiating pentoses from other sugars in various biochemical analyses. It is commonly employed in laboratories for the identification of ribose and other similar sugars in biological samples.

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Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.


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Dehydration of monosaccharide to form furfural http://www.harpercollege.edu/tm-ps/chm/100/dgodambe/thedisk/carbo/bial/bials.htm Formation of furfural/orcinol complex http://61.19.145.8/student/m5year2006-2/502/group08/rooms5.html


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Glucose, fructose, maltose, sucrose, lactose, and starch are carbohydrates that would likely test positive in Molisch, Benedict's, Barfoed's, Seliwanoff's, Bial's, and fermentation tests. These carbohydrates are commonly used in biochemical testing due to their distinct reactivity with the specific reagents in each test.


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