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The ninhydrin test is positive for all proteins because it reacts with free amino groups found in the amino acids that make up proteins. When ninhydrin is applied, it forms a colored complex, typically purple or blue, indicating the presence of amino acids. Since all proteins consist of amino acids linked by peptide bonds, the test detects these amino groups, leading to a positive result for any protein. This makes the ninhydrin test a general method for assessing the presence of proteins in a sample.

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1mo ago

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All living cells would test positive for amino acids?

Yes, all living cells contain amino acids, which are the building blocks of proteins. Proteins are essential for various biological processes, such as enzyme catalysis, structural support, and cell signaling. Therefore, a test for amino acids would likely be positive in all living cells.


Why ninhydrin shows positive test for amino acid and protein?

Ninhydrin reacts with ammonia, a primary amine, or a secondary amine (amino acids have a primary or alpha amino group, except for proline which has a secondary amino group). They all turn purple/blue right away upon heating with ninhydrin.Remember that a protein has a amino terminal and a -COOH terminal. The ninhydrin will react with the amino terminal giving a very light blue or violet color (more often than not you will see no color change). Upon further heating you may notice an increase in the intensity of the blue/violet. this is due to the heat denaturing the protein, thereby exposing more -NH2 groups for the ninhydrin reagent to react with


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