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There was no Charles 1 that ever ruled the Holy Roman Empire. There was a Charles the Great, King of the Franks, Charles the Bald, Charles the Fat, Charles IV of Luxembourg and Charles V that had the title but never a Charles 1.
yes
YES
The King of Spain in 1525 was King Ferdinand who ruled from 1516 to 1555. His wife was his first cousin who he married in 1525 and her name was Isabella.
um i don't know