See the two Related Questions to the left for complete instructions on how to make any solution of any concentrations.
To make a 1 in 4 dilution, it is best is to use a graduated cylinder. Measure out 3 parts water and add to that 1 part stock solution, which gives a total of 4 parts. For instance, if you measure out 30 mL of water, and then add 10 mL of stock solution to that, you will have a total of 40 mL solution, 1/4 the concentration of the original stock solution. (Or, equivalently if you need more than this, measure out 150 mL of water and add 50 mL of stock solution to give a total of 200 mL of the diluted solution). If you don't have a graduated cylinder, you'll need two containers: one small and one much larger. Fill the small one completely with water, and then pour that into a new (larger) container. Do that two more times (for a total of 3). Then fill the smaller container with stock solution, and add the water. When diluting acids and bases, it is extremely important to add the acid/base to water, and not the other way around! If you want to do a dilution with different numbers, just change the numbers accordingly. For example, if you want a 2 in 5 dilution, add 3 parts water to 2 parts stock solution. When it says "2 in 5" that means two parts stock solution to 5 total parts, so that leaves 3 parts water!
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution
A 100 times dilution of a stock solution means that you are adding 1 part of the stock solution to 99 parts of a diluent (such as water) to make a total volume of 100 parts. This results in a final solution that is 1/100th the concentration of the original stock solution.
1 part of stock+ 2part of dillution
To perform serial dilution in DMSO (Dimethyl sulfoxide) 0.01, you would start by preparing a stock solution of your compound in DMSO at a higher concentration. Then, you would dilute this stock solution using DMSO 0.01 to achieve the desired concentrations for your experiment, following a serial dilution scheme where each subsequent sample is diluted from the previous one. Make sure to mix thoroughly between dilutions to ensure even distribution of the compound.
To make a 0.25 M solution of ammonium sulfate from a stock solution of 6 M, you would need to dilute the stock solution. The dilution equation is C1V1 = C2V2 where C1 and V1 are the concentration and volume of the stock solution, and C2 and V2 are the concentration and volume of the final solution. You would need to set up this equation to calculate the volume of the stock solution needed and then convert that volume to grams using the molar mass of ammonium sulfate.
To make a dilution of guanidine isothiocyanate (GITC), first prepare a stock solution at the desired concentration by dissolving the solid in a suitable buffer or water. Then, to dilute this stock solution, mix a specific volume of the stock with an appropriate volume of diluent, such as phosphate-buffered saline (PBS) or water, following the dilution formula (C1V1 = C2V2), where C1 is the initial concentration, V1 is the volume of the stock solution used, C2 is the final concentration, and V2 is the final volume. Ensure to handle GITC in a fume hood and wear appropriate personal protective equipment, as it is toxic. Always label the final solution with the concentration and date prepared.
Diluting with water will make this solution more neutral, meaning it will slowly increase to 7, which is the pH value of pure water used for this dilution.
it is very easy to prepare working solution from a stock solution we use the formula for this purpose which is: C1V1 = C2V2 C1 is the concentration of the stock solution V1 required volume from the stock solution C2 concentration of the working solution V2 volume of the working solution
To make a 1000-fold dilution, take 1 part of your concentrated solution and mix it with 999 parts of a diluent, such as water or buffer. For example, if you start with 1 mL of the concentrated solution, you would add it to 999 mL of the diluent. Mix thoroughly to ensure homogeneity. This results in a dilution where the original solution is reduced to one-thousandth of its initial concentration.
You can dilute by adding distilled water. When diluting, be sure to add the solution to water several times instead of adding water to the solution (especially if it is highly concentrated).
To make a 5X solution from a 10X stock, you can dilute the 10X stock solution by adding an equal volume of diluent (such as water or buffer) to the original solution. For example, if you have 1 mL of the 10X stock solution, you would add 1 mL of diluent to make a 5X solution.
To make a 5% bleach solution from an 8.25% solution, you can use the dilution formula (C_1V_1 = C_2V_2), where (C_1) is the concentration of the stock solution (8.25%), (V_1) is the volume of the stock solution you need, (C_2) is the desired concentration (5%), and (V_2) is the final volume of the diluted solution. For example, to make 100 ml of a 5% solution, you would calculate (V_1 = \frac{C_2 \times V_2}{C_1} = \frac{5 \times 100}{8.25} \approx 60.61) ml of the 8.25% solution. Then, add enough water to reach a total volume of 100 ml.