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The term "endotoxin" is a bit wonky. Originally, it was intended solely to mean a toxin(s) not secreted by the bacterium cell until after cell death. Since then, however, the term has come to refer to the cell wall components of Gram-negative bacteria, most specifically lipopolysaccharide (LPS).

In the case of the term "endotoxin" referring to LPS, then, no -- LPS is a sole toxin (an "A"). However, when the more general meaning of the term "endotoxin" is used, the toxin released may be an A-B toxin.

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What does the toxin Endotoxin do?

The amount of endotoxin in protein, peptide or antibody samples is measured by the Limulus Amebocyte Lysate (LAL) test with an endotoxin kit. The concentration of endotoxin in the test specimens is calculated by the LAL kit by chromogenic signal in the presence of endotoxin. This measurement is performed on a microplagin absorption reader at 405 nm. Protein and antibody samples are measured every 30 minutes. The importance of determining the level of endotoxin in evaluating the effectiveness of endotoxin removal methods as well as preventing endotoxic shocks and animals injected with endotoxin-contaminated proteins is very important.


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