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there are two errors may occur in this sitution.

1.mechanical error.

1.labour error.

ps. standard addition is an exceptional case.

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13y ago

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What is the relationship between absorbance and transmittance?

Absorbance = -log (percent transmittance/100)


How do you calculate Mean Generation Time?

g=(log Nt- log Nto)/log 2 where N=absorbance reading @ time indicated MGT= (t-to)/g


What does the negative absorbance show?

Negative absorbance values typically indicate that the measured sample has lower light absorption than the baseline or reference. This can occur due to factors such as instrument noise, incorrect baseline correction, or interference from other substances. In some cases, it may suggest the presence of scattering or fluorescence rather than true absorbance. Negative absorbance values should be interpreted cautiously and may require further investigation to clarify the underlying cause.


What is difference between specific absorbance and absorbance interms of spectroscopy?

specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com


What is the difference between absorbance and transmittance?

Absorbance measures the amount of light absorbed by a sample, while transmittance measures the amount of light that passes through a sample. Absorbance is calculated as -log(T), where T is transmittance. Absorbance is commonly used in spectrophotometry to quantify the concentration of a substance in a solution.


Why spectophotometer gives negative value?

A spectrometer can provide absorbance information in a number of ways.. For example transmissivity can be given as a percentage value. The absorbance is often represented on a log scale. It may be calculated by: A = -log10(I/I0) Since incident light intensity must be greater than detected light intensity, Absorbance technically can't be negative. However, a spectrometer must be zeroed before each use to provide a baseline. If a material which is contaminated or otherwise inappropriate is used to zero the spectrometer, it may give a bad baseline, and thus a sample may appear to give negative absorbance. The 'blank' which was used to zero the spectrometer would have had higher absorptivity than the sample. Another instance in which you may get negative absorption could be fluorescence. If your material is excited by light, it can emit light at a different frequency. Detecting at this frequency may produce negative absorption. Similarly, 'upconversion' by certain porphyrins may also cause emission at certain wavelengths.


What is -log equivalent to?

The negative log of a number is the log of the number's reciprocal ('1' divided by the number).


How is it possible to find the negative log explain?

Logs are defined only for positive numbers so the log of a negative number does not exist.


Is wavelength or absorbance the dependent variable?

"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.


What is a negative logarithm?

A logarithm of a reciprocal. For example, log(1/7) or log(7-1) = -log(7)


If you have a column of values on excel wth negative and positive values and you want to take the LOG of them what do you do?

You can't take the log of negative numbers - at least, not while you stay in the realm of real numbers.You can't take the log of negative numbers - at least, not while you stay in the realm of real numbers.You can't take the log of negative numbers - at least, not while you stay in the realm of real numbers.You can't take the log of negative numbers - at least, not while you stay in the realm of real numbers.


What is the purpose of the blank?

Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.