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BOD (Biochemical Oxygen Demand) bottles are incubated for 5 days at 20°C to allow microbial activity to decompose organic matter in the sample. This incubation period provides a standardized timeframe for measuring the oxygen consumed by microorganisms, which is indicative of the organic pollution level in the water sample. The 5-day duration is a balance between allowing sufficient microbial action while minimizing variability in results due to factors like temperature fluctuations or microbial growth limits.

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1w ago

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