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What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
Is crystal violet used as carcinogenic agent?
Crystal violet is not classified as a known carcinogen, but it has been shown to have genotoxic and mutagenic properties in some studies. It is important to handle crystal violet with caution and follow proper safety protocols when working with this compound in the lab.
What color would the bacterial cell have when viewed microscopically after the treatment with ammonium oxalate crystal violet?
The bacterial cell would appear purple when viewed microscopically after treatment with ammonium oxalate crystal violet. The crystal violet binds to the peptidoglycan layer of the cell wall, causing it to retain the purple color even after subsequent washing steps.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
What is the charge of crystal violet?
The charge of crystal violet is positive.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
Is the crystal violet test result positive or negative?
The crystal violet test result is positive.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What does crystal violet bind to in biological systems?
Crystal violet binds to nucleic acids, specifically DNA, in biological systems.
What is gentian violet?
Traditional antifungal drugs such as.gentian violet (also known as crystal violet)
What is the extinction coefficient of crystal violet?
The extinction coefficient of crystal violet is approximately 89,000 M^(-1)cm^(-1) at a wavelength of 590 nm. This value indicates the molar absorptivity of crystal violet at this specific wavelength, which is commonly used for measuring the concentration of crystal violet in solution using spectrophotometry.
Does crystal violet have a color?
Yes, crystal violet is a triarylmethane dye that appears as a deep purple color when dissolved in water or other solvents.
Is crystal violet considered a primary stain in the Gram staining technique?
Yes, crystal violet is considered a primary stain in the Gram staining technique.
How many grams are in a 50000 kg crystal?
There are 50,000,000 grams in a 50,000 kg crystal. This is because there are 1,000 grams in 1 kilogram.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
