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Is food poisoning negative or positive in a Gram stain?
There are a variety of bacteria that can cause foodborne illness: some are Gram-positive, others are Gram-negative. See Related Links.
Is it feasible to determine motility using a side prepared by the Gram Stain techniques?
No, motility cannot be determined using the Gram stain technique. The Gram stain method is primarily used to differentiate bacteria based on their cell wall composition (Gram-positive or Gram-negative) and does not provide information on motility. Motility is typically assessed through techniques such as microscopy, agar stab, or hanging drop methods.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
Performing a spore stain is necessary because simple staining may show the presence of spores but does not provide enough contrast to clearly distinguish them from the rest of the cell. Spore staining uses specific dyes and techniques to highlight and differentiate spores from the surrounding cell material, providing clearer visualization and identification of spores.
What is the gram stain for leptospira icterorrhsgiae?
Leptospira as a genus has characteristics of both Gram negative and Gram positive bacteria, in that they have a double membrane with peptidoglycan attached to the inner membrane. Thus when stained they could appear either positive or negative, if one could even see them at all. This in addition to their extreme slenderness makes visualization by gram stain less than ideal. Typically one would observe this organism by darkfield microscopy rather than attempt a likely to fail Gram stain.
What will you see of a gram stain of e coli?
E. coli is a gram negative bacteria, meaning that it has a cytoplasmic lipid membrane, a peptidoglycan layer, and a (LPS) lipopolysaccharide layer. As a result, e. coli stains a pink colour on a gram stain from the counterstain saffranin. Gram positives stain purple retain the crystal violet dye even after washed with a decolouring solution.
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
Is food poisoning negative or positive in a Gram stain?
There are a variety of bacteria that can cause foodborne illness: some are Gram-positive, others are Gram-negative. See Related Links.
Of what use is the endospore stain?
The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
Is it feasible to determine motility using a side prepared by the Gram Stain techniques?
No, motility cannot be determined using the Gram stain technique. The Gram stain method is primarily used to differentiate bacteria based on their cell wall composition (Gram-positive or Gram-negative) and does not provide information on motility. Motility is typically assessed through techniques such as microscopy, agar stab, or hanging drop methods.
What is the gram stain for the west nile virus?
There is no Gram stain for the rabies virus - it does not pick up either the stain or the counter-stain and has no official Gram stain status like bacteria do. When scientists are looking at slides of brains to see if an animal was infected with rabies, they use a special immunofluorescent stain made of antibodies against the rabies virus linked to either a vividly colored pigment or a fluorescent pigment. If the rabies virus is present, the antibodies in the stain adhere to the viral particles and then the pigment becomes fixed to the tissue as well, allowing the pathologist to "see" the virus (actually just that the virus is present and approximately where it is at - the virus is too small to see with a standard light microscope).
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Why is it necessary to perform a spore stain when you can see the presence of spores in simple stained cells?
Performing a spore stain is necessary because simple staining may show the presence of spores but does not provide enough contrast to clearly distinguish them from the rest of the cell. Spore staining uses specific dyes and techniques to highlight and differentiate spores from the surrounding cell material, providing clearer visualization and identification of spores.
What is the gram stain for leptospira icterorrhsgiae?
Leptospira as a genus has characteristics of both Gram negative and Gram positive bacteria, in that they have a double membrane with peptidoglycan attached to the inner membrane. Thus when stained they could appear either positive or negative, if one could even see them at all. This in addition to their extreme slenderness makes visualization by gram stain less than ideal. Typically one would observe this organism by darkfield microscopy rather than attempt a likely to fail Gram stain.
What would happen if you decolorized your slide too much on a gram stain?
The purpose of any microbiological stain (gram or otherwise), is to enable visualization of features that would otherwise be clear/invisible in a sample. In this case, the darker crystal violet is used to stain the peptidoglycan cell walls of gram-positive bacteria. Washing the sample with too much ethanol (decolorizing too much) will remove too much stain, making it difficult or impossible to distinguish between gram-negative and gram-positive cells. Only the counterstain that is added after the ethanol wash (safranin, for example) will be visible.(see related links for example descriptions of Gram staining)
Why is counterstaining necessary when using a differential staning technique such as the gram stain?
The counter-stain allows you to see all the structures that were not stained with the primary stain. Without the counter-stain, all you would see is the purple-stained structures (nucleus, some cytoplasmic proteins), but you would have a difficult time observing the cell membrane and many cytoplasmic structures.
Is varicella zoster virus gram negative of gram positive?
Gram-positive and gram-negative refer to the Gram stain used to prepare slides of bacteria for viewing under a light microscope. Viruses are too small to see under a light microscope and have to be prepared differently for viewing under an electron microscope. So the answer to your question is neither
