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In a shake-tube test, the culture type is distributed throughout the entire medium by rapidly rotating the tube between the palms of your hands and then cooled rapidly by immersion in an iced water bath to ensure that the cells remain dispersed after shaking the test tube. It is then inoculated at appropriate temp for appropriate amount of time. The growth that was more abundant toward the surface was either obligate aerobes, microaerophiles, or facultative anaerobes (further determined by actual presence of the microorganism within the medium) whereas the growth that was equal throughout was most likely an aerotolerant anaerobe.

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