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Biotic factors such as the presence of plants, algae, and organisms like mollusks can indirectly influence the concentration of calcium in water hardness by affecting nutrient levels and pH which in turn can impact the solubility of calcium compounds. For example, the uptake of calcium by aquatic plants and algae can reduce the concentration of calcium in the water. Additionally, organisms with exoskeletons or shells can contribute to water hardness by releasing calcium carbonate as they grow and die.

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