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Inducing spore formation in Bacillus megaterium can be achieved by subjecting the bacteria to nutrient limitation, particularly by depleting essential nutrients such as carbon or nitrogen. Additionally, exposing the culture to stress conditions, such as high temperatures or osmotic pressure, can trigger sporulation. Cultivating the bacteria in a suitable sporulation medium, such as a minimal salts solution, can also promote spore formation. It's essential to maintain optimal growth conditions prior to inducing sporulation to ensure a healthy cell population.

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2d ago

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