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The process of gram staining is simple.
1)smear bacteria from pure culture onto slide, heat fix
2)flood with crystal violet (1min)
3)Add iodine (1 min)
4)acid/alcohol wash (1 min)
5)Flood with safranine (1min)
6)Air dry and examine.
These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close).
Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer.
Source(s):
Medical Microbiology
What else can I help you with?
Candida albicans gram positive or gram negative?
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
Is azospirillum a gram positive bacteria?
Gram positive
What colors gram positive and gram negative bacteria?
Gram-positive cells are purple and the Gram-negative cells are red.
Are archaebacteria gram positive or gram negative?
Archaebacteria are neither gram positive nor gram negative because they do not have peptidoglycan in their cell walls like bacteria. Instead, they have unique cell wall structures that make them distinct from both gram positive and gram negative bacteria.
How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Candida albicans gram positive or gram negative?
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
What bacteria is a gram negative?
One example of a gram-negative bacteria is Escherichia coli (E. coli). Gram-negative bacteria have a cell wall structure that includes an outer membrane, which is not present in gram-positive bacteria. This outer membrane contains lipopolysaccharides that contribute to the bacteria's resistance to certain antibiotics. Gram staining is a common technique used to differentiate between gram-negative and gram-positive bacteria based on their cell wall composition.
Is klebsiella gram positive or negative?
i believe it is a gram bacteria which causes respiratory illness.
Is azospirillum a gram positive bacteria?
Gram positive
What is the advantage of the gram stain over the simple stain?
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
What is the structural difference in the cell walls of gram-negative bacteria that distinguishes them from gram-positive bacteria?
Gram-negative bacteria have a thinner peptidoglycan layer in their cell walls compared to gram-positive bacteria. Additionally, gram-negative bacteria have an outer membrane that contains lipopolysaccharides, which is absent in gram-positive bacteria.
Is gram positive bigger than gram negative?
Gram-positive bacteria and Gram-negative bacteria are different in their colouration when dyed and viewed with a light microscope. Gram-positive appear violet, and Gram-negative appear red. Gram-positive and Gram-negative classification, however, has nothing to do with size.
Name the different gram positive and gram negative bacteria?
Name of gram positive and gram negative bacteria?
Does Tetracycline kill gram positive or gram negative bacteria?
Tetracycline kills both gram positive and gram negative bacteria. The mode of action to which Tetracycline works to kill bacteria is that it inhibits protein synthesis which works against both gram positive and gram negative bacteria.
What are cheek cells gram positive or gram negative?
Cheek cells do not have a cell wall, therefore they are neither gram positive nor gram negative. Gram staining is a technique used to differentiate bacteria based on the composition of their cell wall, which animal cells like cheek cells do not possess.
Is acid fast bacteria gram positive or negative?
Acid-fast bacteria are gram-positive.
What colors gram positive and gram negative bacteria?
Gram-positive cells are purple and the Gram-negative cells are red.
