The process of gram staining is simple.
1)smear bacteria from pure culture onto slide, heat fix
2)flood with crystal violet (1min)
3)Add iodine (1 min)
4)acid/alcohol wash (1 min)
5)Flood with safranine (1min)
6)Air dry and examine.
These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close).
Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer.
Source(s):
Medical Microbiology
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
Gram positive
Gram-positive cells are purple and the Gram-negative cells are red.
Archaebacteria are neither gram positive nor gram negative because they do not have peptidoglycan in their cell walls like bacteria. Instead, they have unique cell wall structures that make them distinct from both gram positive and gram negative bacteria.
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
One example of a gram-negative bacteria is Escherichia coli (E. coli). Gram-negative bacteria have a cell wall structure that includes an outer membrane, which is not present in gram-positive bacteria. This outer membrane contains lipopolysaccharides that contribute to the bacteria's resistance to certain antibiotics. Gram staining is a common technique used to differentiate between gram-negative and gram-positive bacteria based on their cell wall composition.
i believe it is a gram bacteria which causes respiratory illness.
Gram positive
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
Gram-negative bacteria have a thinner peptidoglycan layer in their cell walls compared to gram-positive bacteria. Additionally, gram-negative bacteria have an outer membrane that contains lipopolysaccharides, which is absent in gram-positive bacteria.
Gram-positive bacteria and Gram-negative bacteria are different in their colouration when dyed and viewed with a light microscope. Gram-positive appear violet, and Gram-negative appear red. Gram-positive and Gram-negative classification, however, has nothing to do with size.
Name of gram positive and gram negative bacteria?
Tetracycline kills both gram positive and gram negative bacteria. The mode of action to which Tetracycline works to kill bacteria is that it inhibits protein synthesis which works against both gram positive and gram negative bacteria.
Cheek cells do not have a cell wall, therefore they are neither gram positive nor gram negative. Gram staining is a technique used to differentiate bacteria based on the composition of their cell wall, which animal cells like cheek cells do not possess.
Acid-fast bacteria are gram-positive.
Gram-positive cells are purple and the Gram-negative cells are red.