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To calculate the molecular weight of a protein in electrophoresis, you would use a standard curve generated with protein standards of known molecular weights run on the same gel. By plotting the migration distance of the standard proteins against their known molecular weights, you can then determine the molecular weight of your protein of interest based on its migration distance on the gel in comparison to the standard curve.
What else can I help you with?
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
How to calculate empirial formula from molecular formula?
To calculate the empirical formula from a molecular formula, divide the subscripts in the molecular formula by the greatest common factor to get the simplest ratio of atoms. This simplest ratio represents the empirical formula.
How do you calculate the molecular weight and the uncertainty of the molecular weight of a unknown solute using propagated error?
To calculate the molecular weight, you need to perform a chemical analysis to determine the elemental composition of the unknown solute. Then, using the atomic masses of the elements found, you can calculate the molecular weight by summing the atomic weights of all the atoms in the molecule. To determine the uncertainty in the molecular weight, you would need to propagate the errors in the atomic masses and the elemental composition through the calculation using the rules of error propagation.
How do you calculate the molecular weight of KCl?
The atomic weight of potassium is 39,0983 g.The atomic weight of chlorine is 25,45 g.The molecular weight of potassium chloride (K + Cl) is 74,5483 g.
How do you calculate molecular weight of tio2?
Ti = 1 x 47.867 = 47.867 O = 2 x 15.999 = 31.998 -------------------------------- Molecular weight = 79.865g/mol
Why do you need a molecular weight ruler alongside your samples during electrophoresis?
A molecular weight ruler uses a sample of fragments of a known size (known as a molecular weight marker) to be placed alongside the experimental and control samples. It helps compare the migration distance of the experimental fragments to the migrating distance of the fragments of a known size that make up the molecular weight marker. Then the scientist can calculate an approx. size of their experimental samples.
Will two molecules having the same molecular weight and charge have the same mobility in electrophoresis?
yes...!
One lane of an electrophoresis gel shows a 100bp ladder. What is the purpose of this ladder?
It is used as a marker for molecular weight.
What is the process to calculate the gram molecular weight of the unknown liquid?
To calculate the gram molecular weight of an unknown liquid, you need to determine the molecular formula of the liquid and then add up the atomic weights of all the atoms in the formula. This will give you the gram molecular weight of the unknown liquid.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
Who discovered SDS PAGE electrophoresis?
SDS-PAGE electrophoresis was developed by biochemist Ulrich K. Laemmli in 1970. It is a widely used technique for separating proteins based on their molecular weight.
How to calculate empirial formula from molecular formula?
To calculate the empirical formula from a molecular formula, divide the subscripts in the molecular formula by the greatest common factor to get the simplest ratio of atoms. This simplest ratio represents the empirical formula.
How do you calculate equivalent weight of oxide?
To calculate the equivalent weight of an oxide, you first determine its molecular weight. Then you look at how many oxygen atoms are present in the formula of the oxide and divide the molecular weight by that number of oxygen atoms. This result is the equivalent weight of the oxide.
How do you calculate number average molecular weight given molecular weight range and fraction?
To calculate the number average molecular weight, multiply each molecular weight value within the range by its corresponding fraction, then sum up these products. This sum represents the numerator of the equation. Next, sum up all the fractions in the denominator. Finally, divide the numerator by the denominator to obtain the number average molecular weight.
How do you calculate the molecular weight and the uncertainty of the molecular weight of a unknown solute using propagated error?
To calculate the molecular weight, you need to perform a chemical analysis to determine the elemental composition of the unknown solute. Then, using the atomic masses of the elements found, you can calculate the molecular weight by summing the atomic weights of all the atoms in the molecule. To determine the uncertainty in the molecular weight, you would need to propagate the errors in the atomic masses and the elemental composition through the calculation using the rules of error propagation.
How do you calculate the molecular weight of KCl?
The atomic weight of potassium is 39,0983 g.The atomic weight of chlorine is 25,45 g.The molecular weight of potassium chloride (K + Cl) is 74,5483 g.
How do you calculate molecular weight of tio2?
Ti = 1 x 47.867 = 47.867 O = 2 x 15.999 = 31.998 -------------------------------- Molecular weight = 79.865g/mol
