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Total sugars, if present in foods at a concentration beyond 5 to 10%, it can be determined by the Lane & Eynon's titrimetric method using mixture of Fehling A and Fehling B solutions. Since total sugars are a mixture of reducing sugars and non-reducing sugars, its determination involves two steps.
Step 1. To a known weight of the sample, about 80 ml of warm water (50 deg C) is added and mixed. It is then made to known volume and filtered. Transfer the filtrate to a burette. Take 5ml of Fehling A and Fehling B, each in a conical flask and mix well. Add about 15 ml distilled water and keep on a hot plate to boil the contents. Run 15 ml of solution from the burette into the flask. Allow to boil for one minute. Add methylene blue indicator (1% solution in water, 5 drops) and continue titration till you get a brick red end point. Note down the titer value. Using the Fehling's factor, calculate the reducing sugars present in the sample.
Step 2. Using the same procedure determine the total sugars after hydrolysing the solution with 5 ml conc. Hydrochloric acid by keeping the solution overnight. Neutralize the solution with NaOH using phenolphtalein indicator. make up to know volume. Transfer the solution to burette and titrate against Fehling A+B solution.
Calculate the total sugars from this titer value.
What else can I help you with?
What group of substance that give positive reaction to molisch test?
Carbohydrates typically give a positive reaction to the Molisch test. This is because the Molisch reagent reacts with the carbohydrates to form a purple complex, indicating the presence of sugar molecules in the solution.
What does Benedicts plus sugar equal?
Benedict's solution plus sugar is used in the Benedict's test to measure the presence of reducing sugars. The sugar reacts with the copper ions in Benedict's solution, forming a colored precipitate that indicates the presence of reducing sugars. This test is commonly used to detect the presence of glucose and maltose in various biological samples.
Why doesn't polysaccharides change in the presence of Benedict's solution?
Benedict's test is a test used to determine the presence of reducing sugars. Sucrose is not a reducing sugar that's why its color doesn't change. . .
How do you determine a strach?
A starch is a complex carbohydrate made up of long chains of sugar molecules. It can be determined using various chemical tests such as iodine test, which turns blue-black in the presence of starch, or by using enzymatic methods to break down starch into individual sugar units for quantification.
How do you test for sugar in foods?
One common method to test for sugar in foods is using a glucose meter, which can provide a quantitative measure of sugar content. Another method is using a chemical test, such as the Benedict's test, which involves adding a reagent that changes color in the presence of reducing sugars like glucose. Additionally, specific laboratory tests can be conducted to measure sugar content in a more detailed and accurate manner.
What does Benedict's Test provide or test for?
Benedict's test estimates on color scale the presence of sugar .Normally used as a random pathological chemical test to determine the presence of sugar in urine in diabetes patients.In presence of sugar Benedict's solution turns from blue to shades of yellow,red,brown & dark according to the percentage of sugar present in solution on heating the solution.
What is ammonia used to test for copper in a solution?
Ammonia is used to test for the presence of copper in a solution because it forms a deep blue complex with copper ions. This complex is known as Schweizer's reagent and can be used as a qualitative test for the presence of copper ions.
What group of substance that give positive reaction to molisch test?
Carbohydrates typically give a positive reaction to the Molisch test. This is because the Molisch reagent reacts with the carbohydrates to form a purple complex, indicating the presence of sugar molecules in the solution.
Why do all sugar give a positive molisch's test?
All sugars give a positive Molisch's test because they contain hydroxyl (–OH) groups that can react with α-naphthol in the presence of sulfuric acid. This reaction leads to the formation of a purple-colored complex, indicating the presence of carbohydrates. Both monosaccharides and disaccharides, which are classified as carbohydrates, can participate in this reaction, resulting in a positive test. Thus, the test serves as a general indicator for the presence of sugars.
Why does the Diphenylamine test only react with DNA?
The Diphenylamine test is specific to DNA because it reacts with the deoxyribose sugar present in the DNA backbone. The test detects the presence of DNA by forming a blue color complex with the deoxyribose, which is unique to DNA and not found in other nucleic acids like RNA.
What does Benedicts plus sugar equal?
Benedict's solution plus sugar is used in the Benedict's test to measure the presence of reducing sugars. The sugar reacts with the copper ions in Benedict's solution, forming a colored precipitate that indicates the presence of reducing sugars. This test is commonly used to detect the presence of glucose and maltose in various biological samples.
Why doesn't polysaccharides change in the presence of Benedict's solution?
Benedict's test is a test used to determine the presence of reducing sugars. Sucrose is not a reducing sugar that's why its color doesn't change. . .
What is I2KI used to test for?
I2KI (iodine/potassium iodide solution) is used to test for the presence of starch. In the presence of starch, the solution will turn blue-black due to the formation of a starch-iodine complex.
How do you test a diet soda to check if it contains sugar - slide5?
To test a diet soda for sugar, you can use a simple approach with a reagent like Benedict's solution. Heat a small amount of the soda with the reagent; if sugar is present, the solution will change color, indicating the presence of reducing sugars. Alternatively, you can use a refractometer or test strips designed for sugar detection. Remember that diet sodas typically contain artificial sweeteners instead of sugar, so a positive test may suggest the presence of residual sugars or other ingredients.
How do you determine a strach?
A starch is a complex carbohydrate made up of long chains of sugar molecules. It can be determined using various chemical tests such as iodine test, which turns blue-black in the presence of starch, or by using enzymatic methods to break down starch into individual sugar units for quantification.
Is oxygen needed for the metabolism of sugar?
Yes...if processing is done aerobically...(most complex organisms, like mammals). Anaerobes do not require the presence of oxygen to metabolize sugar.
Why purple colour give in Molisch test?
The purple color in the Molisch test is due to the formation of a complex between the carbohydrate present in the sample and the alpha-naphthol reagent used in the test. This complex formation indicates the presence of carbohydrates in the sample.
