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diphenylamine binds with the deoxyribose molecule to form a blue complex
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∙ 13y ago
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What is diphenylamine test for deoxyribose for?
When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution
DNA estimation by DPA method?
in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
What are the Chemicals that react with DNA?
yes
Can a woman get a blood test and find out the unborn babys DNA?
No the blood test will determine the DNA of the mother not the DNA of the baby
Where is the DNA test centers in India?
In Hyderabad, OTS & Services (DNA Test India)
What is diphenylamine test for deoxyribose for?
When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution
When does a spray of diphenylamine reagent on TLC gives blue coloration?
Diphenylamine is a reagent for DNA.
Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
What are the methods for estimation of DNA?
diphenylamine reagent
Diphenylamine will turn blue in the presence of?
DNA
What will diphenylamine turn blue in the prescense of?
DNA
DNA estimation by DPA method?
in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
How do you get negative paternity test?
That's a DNA test, it will only be negative if you are not the parent.
How do you use diphenyl amine to estimate DNA?
Diphenyle amine reacts with DNA and liberates inorganic ortho phoshate of Dna. amine group of diphenylamine is combined with DNA before the phosphate group is released from the sugar. By this action DNA solution gets dark blue color which was estimated by using spectrophotometer.
How do you know that your goldfish is a boy?
Only a dna test can determine.
How can one know his child without DNA test?
If you don't trust the mother a DNA test is the only way I guess. The child can also look like you.
How many ways can DNA be misread?
if someone had taken a DNA test when a child was about 6 months old only to find out that he was the Dad 4 years later. Is this possible or should he get another DNA test done?
