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When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution
Wiki User
∙ 12y ago
Wiki User
∙ 13y agoOne major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. DNA can be identified chemically with the Dische diphenylamine test. The reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex
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Why does the Diphenylamine test only react with DNA?
diphenylamine binds with the deoxyribose molecule to form a blue complex
DNA estimation by DPA method?
in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
Which sugar present in DNA?
Deoxyribose sugar, it is a pentose sugar base.
What Sugar used in DNA to make up the backbone?
Deoxyribose sugar, it is a pentose sugar base.
What is the 5-carbon sugar found in a nucleotide?
it is deoxyribose. there is little difference between ribose and deoxyribose though.
Why does the Diphenylamine test only react with DNA?
diphenylamine binds with the deoxyribose molecule to form a blue complex
Why does blue colour appears in diphenylamine test?
Blue color appears in diphenylamine test because of the reaction of nitrates.
Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
How to prepare nitrate test solution using diphenylamine in sulphuric acid?
Mix 90ml. sulfuric acid with 10ml. water, to this add 0.5gm. diphenylamine
WHAT IS TEST REAGENT USED TO IDENTIFY NITRATE ION?
A common test for nitrate ion is with diphenylamine.
When does a spray of diphenylamine reagent on TLC gives blue coloration?
Diphenylamine is a reagent for DNA.
DNA estimation by DPA method?
in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
Is diphenylamine organic or inorganic?
organic
What are the methods for estimation of DNA?
diphenylamine reagent
Diphenylamine will turn blue in the presence of?
DNA
What will diphenylamine turn blue in the prescense of?
DNA
Which sugar present in DNA?
Deoxyribose sugar, it is a pentose sugar base.
