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Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
Why must proteins must be removed before quantitative analysis of DNA by the Dische reaction?
Because dna is most of the time coiled around several types of proteins called histones and have interactions with other proteins necessary in the formation of the chromosomes. Also some dna such as in bactyerial cells, ribosomal dna are simultaneously attached to the circular dna during translation and transcription.
What is diphenylamine test for deoxyribose for?
When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution
Does water react with diphenylamine?
One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra
When was Irene Dische born?
Irene Dische was born in 1952.
How do you say dork in German?
it's pronounced alt. mo. dische(sh-e)
Why must proteins must be removed before quantitative analysis of DNA by the Dische reaction?
Because dna is most of the time coiled around several types of proteins called histones and have interactions with other proteins necessary in the formation of the chromosomes. Also some dna such as in bactyerial cells, ribosomal dna are simultaneously attached to the circular dna during translation and transcription.
What is dische test?
A dische test is a timed color-producing method which is based on a reaction of Ru5P with carbazole and L-cysteine in the presence of a strong acid to generate a beautiful pink compound with an absorbance at 546 nm.
What actors and actresses appeared in Klimawechsel - 2009?
The cast of Klimawechsel - 2009 includes: Rainer Bock as Psychiater Sarah Camp Karin Engelhard Anton Figl Christopf Frenner Ulla Geiger as Messieberaterin Maria Happel as Angelika Arndt Leopold Hornung Stefan Hunstein as Galerist Alissa Kaeser Evi Kehrstephan as Ina Illner Horst Kotterba as Kurt Arndt Ulrike Kriener as Beate Busch Maren Kroymann as Dr. Evelyn Bach Thomas Loibl Eva Maria Bayerwaltes as Ronnies Mutter Andi Niessner Maximilian Pelz as Florian Fels Felix Rech as Tantralehrer Andrea Sawatzki as Desiree Dische Tanja Schleiff Kai Schumann as Ronnie Dische Pablo Sprungala as Olaf Oliver Stokowski as Oliver Busch Janina Stopper as Miriam Busch Sophie von Kessel as Hanna Hecht Stefanie von Poser Diego Wallraff as Gustavo Rudolf Wessely Yvonne Yung Hee as Li Mehmet Zengin David Zimmerschied as Sven Bach August Zirner as Dr. Dieter Dumont Christoph Zrenner as Herr Fels
What is the indicator for nucleic acids?
Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl). This method requires the following reagents 1. Orcinol reagent : 6% orcinol in 95% ethanol. 2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.)) Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test. This method requires the following reagents one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization. Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution. This method requires the following reagents Hydrochloric acid, 1Normal Schiff's reagent (made from pararosanilin treated with sulphurous acid) Light green, 1% aqueous (can be replaced with Fast green FCF)
What is the testing indicator for nucleic acids?
For nucleic acids, commonly used testing indicators include ethidium bromide and SYBR Green, which fluoresce when bound to DNA or RNA, allowing visualization under ultraviolet light. These indicators are used in techniques like agarose gel electrophoresis and quantitative PCR to detect and quantify nucleic acids.
What actors and actresses appeared in Druckfrisch - 2003?
The cast of Druckfrisch - 2003 includes: Mario Adorf as himself Manuel Andrack as himself Louis Begley as himself Wolf Biermann as himself Silvia Bovenschen as herself Emma Braslavsky as herself Michael Crichton as himself Irene Dische as herself Jeffrey Eugenides as himself Joschka Fischer as himself Wolf Haas as himself Nikolaus Heidelbach as himself Thomas Hettche as himself Felicitas Hoppe as herself Walter Kempowski as himself Vincent Klink as himself Alexander Kluge as himself Donna Leon as herself Verena Lueken as herself Hans Magnus Enzensberger as himself Henning Mankell as himself Andrea Maria Schenkel as herself Petros Markaris as himself Martin Mosebach as himself Jan Philipp Reemtsma as himself Dieter Richter as himself Salman Rushdie as himself Denis Scheck as Himself - Host Raoul Schrott as himself Peter Sloterdijk as himself Zadie Smith as herself Burkhard Spinnen as himself Heinrich Steinfest as himself Ilija Trojanow as himself Martin Walser as himself Wendelin Wiedeking as himself Feridun Zaimoglu as himself
What movie and television projects has Andrea Sawatzki been in?
Andrea Sawatzki has: Played Anita Joest in "Tatort" in 1969. Performed in "Tatort" in 1969. Played Alice Rains in "Tatort" in 1969. Played Dr. Natascha Severin in "Tatort" in 1969. Played Gabi Bauer in "Polizeiruf 110" in 1971. Played Gabi in "Polizeiruf 110" in 1971. Played Gabriele Zimmermann in "Polizeiruf 110" in 1971. Played herself in "III nach neun" in 1974. Performed in "Auf Achse" in 1978. Played herself in "NDR Talk Show" in 1979. Performed in "Der Fahnder" in 1984. Played Silvia in "Florian" in 1990. Played herself in "Boulevard Bio" in 1991. Played herself in "Riverboat - Die MDR-Talkshow aus Leipzig" in 1992. Played Elke Jansen in "Wolffs Revier" in 1992. Played Frau Harder in "Rosa Roth" in 1994. Played Kerstin in "Faust" in 1994. Played Angelika Mommsen in "Bella Block" in 1994. Played Selma Melle-Schneiders in "Kommissar Rex" in 1994. Played Vera Menzel in "Schwarz greift ein" in 1994. Played Tanja Engel in "Schwurgericht" in 1995. Played Sonja Hersfeld in "A.S." in 1995. Performed in "Angst" in 1995. Played herself in "Die Harald Schmidt Show" in 1995. Played Sonja in "A.S." in 1995. Played Olga Bono in "Die Mutter des Killers" in 1996. Played Elvira in "Atemlos durch die Nacht" in 1996. Played Tamara in "Tresko - Im Visier der Drogenmafia" in 1996. Played Eva Brack in "Agentenfieber" in 1997. Played Iris Dohmann in "Champagner und Kamillentee" in 1997. Played Alma Siebert in "Die Apothekerin" in 1997. Played Yvonne Jacobi in "Falsche Liebe" in 1997. Played Astrid in "Eiskalte Liebe" in 1997. Played Ludwig in "Bandits" in 1997. Played Tanja Engel in "Der Mordsfilm" in 1997. Played Sylvia in "Das Leben ist eine Baustelle." in 1997. Played Gloria in "Buy 1 Get 1 Free" in 1997. Played Erika Relin in "Die Cleveren" in 1998. Played Dr. Patricia Marr in "Der letzte Zeuge" in 1998. Played Elke Spiekermann in "Tod auf Amrum" in 1998. Performed in "Das Experiment" in 1998. Played Steffi Kaminski in "Und alles wegen Mama" in 1998. Performed in "Mordkommission" in 1998. Played herself in "Die Johannes B. Kerner Show" in 1998. Played Kerstin in "Hund mit t" in 1998. Played Wanda Nissen in "Der letzte Zeuge" in 1998. Played herself in "Beckmann" in 1999. Performed in "Doppeltes Dreieck" in 1999. Played Sonja Vogel in "Late Show" in 1999. Played Helen in "Apokalypso - Bombenstimmung in Berlin" in 1999. Played Frl. Wiesenthal in "Klemperer - Ein Leben in Deutschland" in 1999. Played Anna in "Verzweiflung" in 2000. Played Tante Zelda in "Harte Jungs" in 2000. Played Sushiesserin in "Alles mit Besteck" in 2000. Played Pamela Wedekind in "Unterwegs zur Familie Mann" in 2001. Played Pamela Wedekind in "Die Manns - Ein Jahrhundertroman" in 2001. Played Angela Blume in "Bargeld lacht" in 2001. Played Helga Steinheil in "Leo und Claire" in 2001. Played Cora in "Nur mein Sohn war Zeuge" in 2001. Played Britta in "Das verflixte 17. Jahr" in 2002. Played Malise in "Der Mann von nebenan" in 2002. Played Sabine Kittler in "Der Unbestechliche" in 2002. Played herself in "Die Hit-Giganten" in 2003. Played herself in "Die ultimative Chartshow" in 2003. Played Sonja Maiwald in "Mein erster Freund, Mutter und ich" in 2003. Played Herself - Team Orange in "Star Biathlon" in 2005. Played Dr. Helen Cordes in "Helen, Fred und Ted" in 2006. Played Katharina Giano in "Das Schneckenhaus" in 2006. Played herself in "Wir sind Kaiser" in 2007. Played herself in "Dickes B." in 2007. Played Evchen Morell in "Der andere Junge" in 2007. Played Veronika Vermont in "Der Baum" in 2007. Played Anne Bergmann in "Vom Atmen unter Wasser" in 2008. Played herself in "Markus Lanz" in 2008. Played Desiree Dische in "Klimawechsel" in 2009. Played Zelda in "Je ne vous oublierai jamais" in 2010. Played herself in "Deutschland gegen Holland - Das Duell" in 2010. Played Ute Meier-Thiel in "Das grosse Comeback" in 2011. Played Dr. Jutta Grimm in "Visionado obligado" in 2011. Played Adriana De Mila in "Borgia" in 2011. Played herself in "Bayerischer Fernsehpreis" in 2011. Played Bella Jung in "Bella Australia" in 2012. Played Herself - Expertin Literatur in "Der Super-Champion 2012" in 2012. Played Iris in "Meine Tochter, ihr Freund und ich" in 2012. Played Bella Jung in "Bella Familia - Umtausch ausgeschlossen" in 2013. Played Bella Jung in "Bella Dilemma - Drei sind einer zu viel" in 2013.
