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A dische test is a timed color-producing method which is based on a reaction of Ru5P with carbazole and L-cysteine in the presence of a strong acid to generate a beautiful pink compound with an absorbance at 546 nm.

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When was Irene Dische born?

Irene Dische was born in 1952.


What color changes indicate a positive result for dische diphenylamine?

A positive result for dische diphenylamine test would be indicated by a deep blue color change in the solution. This color change indicates the presence of DNA, as dische diphenylamine reacts with deoxyribose sugars in DNA to produce a blue color.


How do you say dork in German?

it's pronounced alt. mo. dische(sh-e)


Does water react with diphenylamine?

One major difference between DNA and RNA is their sugar: DNA contains deoxyribose, whereas RNA contains ribose. This is what isdiphenylamine testfor deoxyribose for. In this process, the reaction between the Dische reagent and 2-deoxypentose results in the development of a blue color. The reaction depends on the conversion of the pentose to w-hydroxylaevulinic aldehyde which then reacts with diphenylamine to give a a blue colored complex. The intensity of the blue color is proportional to the concentration of DNA. Dische reagent does not react with the ribose sugar in RNA and does not form a blue-colored complex.Dische diphenylamine test-- A chemical test utilized to detect the presence of DNA in a substance.Mudit Mishra


What is diphenylamine test for deoxyribose for?

When adding diphenylamine to deoxyribose you will get a dark deep purple color. If you react diphenylamine with crude DNA you will get a pink-violet color. DIphenylamine test is quantitative and the darker the color the greater the concentration of DNA in the solution


Why must proteins must be removed before quantitative analysis of DNA by the Dische reaction?

Because dna is most of the time coiled around several types of proteins called histones and have interactions with other proteins necessary in the formation of the chromosomes. Also some dna such as in bactyerial cells, ribosomal dna are simultaneously attached to the circular dna during translation and transcription.


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